转录因子ZBTB4在甲状腺乳头状癌中的表达及其与肿瘤临床病理特征的关系  

Expression of transcription factor ZBTB4 in papillary thyroid carcinoma and its relationship with clinicopathological features

作　　者：姜琳[1] 席芸 龚万钢 赏金标[1] Jiang Lin;Xi Yun;Gong Wangang;Shang Jinbiao(Department of Thyroid Surgery,Zhejiang Cancer Hospital,Hangzhou 310022,China;Department of Pathology,Zhejiang Cancer Hospital,Hangzhou 310022,China;Biological Specimen Repository,Zhejiang Cancer Hospital,Hangzhou 310022,China)

机构地区：[1]浙江省肿瘤医院甲状腺外科,杭州310022 [2]浙江省肿瘤医院病理科,杭州310022 [3]浙江省肿瘤医院生物样本库,杭州310022

出　　处：《中华内分泌外科杂志(中英文)》2025年第1期63-67,共5页Chinese Journal of Endocrine Surgery

基　　金：浙江省中医药科技计划项目(2023ZL299)。

摘　　要：目的:探讨转录因子锌指蛋白4(ZBTB4)在甲状腺乳头状癌(PTC)中的表达及其与肿瘤临床病理特征的关系。方法:采用免疫组织化学(IHC)方法检测60例PTC患者(其中30例为单纯乳头状癌,30例为伴有桥本氏甲状腺炎(HT)的乳头状癌)手术标本中癌组织和正常组织ZBTB4的表达情况。收集部分患者新鲜组织样本,采用qRT-PCR和Western blot方法检测肿瘤组织中ZBTB4基因mRNA和ZBTB4蛋白的表达情况。采用SPSS统计软件分析该基因在肿瘤组织中的表达水平与临床病理参数及肿瘤预后的关系。结果:免疫组化染色结果显示,PTC患者肿瘤组织中ZBTB4蛋白表达阳性评分高于正常甲状腺组织,差异有统计学意义(25.0%比3.3%,P=0.001)。qRT-PCR结果显示,ZBTB4基因mRNA在肿瘤组织中的表达水平显著高于正常甲状腺组织,差异有统计学意义(P<0.001)。单纯PTC与伴有HT的PTC患者之间肿瘤组织ZBTB4的表达水平无明显差异。多因素分析结果显示,ZBTB4高表达患者肿瘤直径较小(t=2.31,P=0.025)、侧颈部淋巴结转移较少(t=4.13,P=0.042)。结论:ZBTB4高表达患者的临床结局优于低表达患者,体现在肿瘤直径更小、侧颈部淋巴结转移率更低。ZBTB4基因在PTC中的作用机制有待进一步研究。Objective:To investigate the expression of transcription factor Zinc finger and BTB domain-containing 4(ZBTB4)in papillary thyroid carcinoma and its relationship with the clinicopathological features of the tumor.Methods:Immunohistochemistry(IHC)was used to assess ZBTB4 protein of cancerous tissues and paired-normal tissues extracted from surgical samples of 60 patients with papillary thyroid carcinoma(30 classical and 30 with Hashimoto’s thyroiditis).Fresh tissue samples were collected from above patients,and the expression of ZBTB4 gene mRNA and ZBTB4 protein in cancerous tissues was detected by qRT-PCR and Western blot methods.SPSS statistical software was used to analyze the relationship between the expression level of this gene in tumor tissues,clinicopathological features and tumor prognosis.Results:Immunohistochemical results showed that the expression of ZBTB4 protein in papillary thyroid carcinoma tissues was significantly higher than that in normal thyroid tissues(25.0%vs.3.3%,P=0.001).The results of qRT-PCR showed that the expression of ZBTB4 gene mRNA in tumor tissues was significantly higher than that in normal thyroid tissues(P<0.001).There was no significant difference in the expression level of ZBTB4 between patients with classical papillary carcinoma and those with Hashimoto’s thyroiditis.Multivariate analysis showed that the patients with higher expression of ZBTB4 had relatively smaller tumor diameter(t=2.31,P=0.025)and less lateral cervical lymph node metastasis(t=4.13,P=0.042).Conclusions:Patients with higher ZBTB4 expression level have better outcome,as reflected in smaller tumor diameter and lower rates of lateral cervical lymph node metastasis.The molecular mechanism of ZBTB4 gene in papillary thyroid carcinoma needs further study.

关 键 词：转录因子 ZBTB4 甲状腺乳头状癌 桥本氏甲状腺炎 甲状腺 

分 类 号：R736.1[医药卫生—肿瘤]