原儿茶酸对卵巢癌细胞增殖迁移和凋亡的影响和机制研究  

Study on the effect of protocatechuic acid on the proliferation,migration and apoptosis of ovarian cancer cells and its mechanism

作　　者：陈晨晨[1] 姜海珍 陈瑾 王云飞[1] Chen Chenchen;Jiang Haizhen;Chen Jin;Wang Yunfei(Department of Gynecology,Affiliated Hospital of Jining Medical College,Jining 272029,China)

机构地区：[1]济宁医学院附属医院妇科,济宁272029

出　　处：《中华内分泌外科杂志(中英文)》2025年第1期80-84,共5页Chinese Journal of Endocrine Surgery

基　　金：山东省卫计委科研课题基金面上项目(2017WS336)。

摘　　要：目的:探讨原儿茶酸(PCA)对卵巢癌细胞株A2780的生长抑制作用并初步研究其机制。方法:体外培养A2780细胞,随机分为对照组和PCA 5μM和10μM组。以MTT法检测细胞的增殖情况;伤口愈合实验检测细胞迁移能力;流式细胞仪检测细胞周期及凋亡情况;蛋白免疫印迹法检测细胞凋亡相关蛋白的表达。结果:与对照组相比,PCA组(5μM,10μM)48 h吸光度下降,PCA显著抑制细胞增殖(P<0.05);PCA 5μM处理组其G2/M期细胞比例上升至25.26%,10μM处理组G2/M期比例上升至24.99%,PCA组可将人卵巢癌细胞株A2780的细胞周期阻滞在G2/M期;PCA组可促进A2780细胞的早期凋亡率和晚期凋亡率均升高,并导致相关凋亡蛋白CASPASE-3表达水平上调,Bax表达水平上调,而抗凋亡蛋白Bcl2表达水平下调。结论:PCA可抑制卵巢癌细胞株A2780细胞的增殖和迁移能力,阻滞A2780细胞的细胞周期,且促进A2780细胞的凋亡。其作用机制可能与促进凋亡相关蛋白BAX和caspase3的表达有关。Objective:To explore the inhibitory effect of protocatechuic acid(PCA)on the growth of ovarian cancer cell line A2780 and its mechanism.Methods:A2780 cells were cultured in vitro and randomly divided into a control group and protocatechuic acid-treated groups with concentrations of 5μM and 10μM.Cell proliferation was detected using the MTT assay;Cell migration ability was assessed through wound healing experiments;Cell cycle and apoptosis were analyzed using flow cytometry;Protein expression of apoptosis-related proteins was examined using Western blot.Results:Compared with the control group,the absorbance of the protocatechuic acid groups(5μM,10μM)decreased after 48 hours,and protocatechuic acid significantly inhibited cell proliferation(P<0.05).In the 5μM protocatechuic acid treatment group,the proportion of cells in the G2/M phase increased to 25.26%,and in the 10μM treatment group,the proportion of cells in the G2/M phase increased to 24.99%.Protocatechuic acid groups could arrest the cell cycle of the human ovarian cancer cell line A2780 at the G2/M phase.Protocatechuic acid groups could also promote the increase of early and late apoptosis rates of A2780 cells,leading to the upregulation of the expression levels of related apoptotic proteins CASPASE-3 and Bax,while the expression level of the anti-apoptotic protein Bcl2 was downregulated.Conclusions:Protocatechuic acid can inhibit proliferation and migration of A2780 ovarian cancer cells,arrest the cell cycle of A2780 cells,and promote apoptosis.The mechanism of action may be related to the upregulation of apoptosis-related proteins BAX and caspase3.

关 键 词：原儿茶酸 卵巢癌细胞 增殖 凋亡 

分 类 号：R73[医药卫生—肿瘤]