Deciphering the toxic effects of polystyrene nanoparticles on erythropoiesis at single-cell resolution  

作　　者：Eun Jung Kwon Hyeon Mi Sung Hansong Lee Soyul Ahn Yejin Kim Chae Rin Lee Kihun Kim Kyungjae Myung Won Kyu Kim Dokyoung Kim Sanghwa Jeong Chang-Kyu Oh Yun Hak Kim 

机构地区：[1]Medical Research Institute,Pusan National University,Yangsan,Gyeongsangnam-do 50612,Republic of Korea [2]Institute for Future Earth,Pusan National University,Busan 46241,Republic of Korea [3]Department of Convergence Medical Sciences,School of Medicine,Pusan National University,Yangsan,Gyeongsangnam-do 50612,Republic of Korea [4]Department of Biomedical Informatics,School of Medicine,Pusan National University,Yangsan,Gyeongsangnam-do 50612,Republic of Korea [5]Department of Anatomy,School of Medicine,Pusan National University,Yangsan,Gyeongsangnam-do 50612,Republic of Korea [6]Center for Genomic Integrity,Institute for Basic Science,Ulsan 689-798,Republic of Korea [7]Department of Biomedical Engineering,College of Information-Bio Convergence Engineering,Ulsan National Institute of Science and Technology,Ulsan 44919,Republic of Korea [8]Natural Product Research Center,Korea Institute of Science and Technology(KIST),Gangneung,Gangwon-do 25451,Republic of Korea [9]Department of Convergence Medicine,Yonsei University Wonju College of Medicine,Wonju,Gangwon-do 26426,Republic of Korea [10]Natural Product Applied Science,KIST School,University of Science and Technology(UST),Gangneung,Gangwon-do 25451,Republic of Korea [11]Department of Biomedical Science,Graduate School,Kyung Hee University,Seoul 02447,Republic of Korea [12]Department of Anatomy and Neurobiology,College of Medicine,Kyung Hee University,Seoul 02447,Republic of Korea [13]Department of Precision Medicine,Graduate School,Kyung Hee University,Seoul 02447,Republic of Korea [14]Center for Converging Humanities,Kyung Hee University,Seoul 02447,Republic of Korea [15]KHU-KIST Department of Converging Science and Technology,Kyung Hee University,Seoul 02447,Republic of Korea [16]UC San Diego Materials Research Science and Engineering Center,9500 Gilman Drive,La Jolla,California 92093,USA [17]Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute,Core Research Institute(CRI),Kyung Hee University,Seoul 02447,Republic of

出　　处：《Zoological Research》2025年第1期165-176,共12页动物学研究(英文)

基　　金：supported by the Institute for Basic Science (IBS-R022-D1);Global Learning&Academic Research Institution for Master’s/Ph D students and Post-Doc Program of the National Research Foundation of Korea Grant funded by the Ministry of Education (RS-2023-00301938);National Research Foundation of Korea Grant funded by the Korean government (RS-2024-00406152,MSIT);Additional financial support was provided by the 2024 Post-Doc Development Program of Pusan National University,Korea Medical Institute,and KREONET。

摘　　要：Polystyrene nanoparticles pose significant toxicological risks to aquatic ecosystems,yet their impact on zebrafish(Danio rerio)embryonic development,particularly erythropoiesis,remains underexplored.This study used single-cell RNA sequencing to comprehensively evaluate the effects of polystyrene nanoparticle exposure on erythropoiesis in zebrafish embryos.In vivo validation experiments corroborated the transcriptomic findings,revealing that polystyrene nanoparticle exposure disrupted erythrocyte differentiation,as evidenced by the decrease in mature erythrocytes and concomitant increase in immature erythrocytes.Additionally,impaired heme synthesis further contributed to the diminished erythrocyte population.These findings underscore the toxic effects of polystyrene nanoparticles on hematopoietic processes,highlighting their potential to compromise organismal health in aquatic environments.

关 键 词：Polystyrene nanoparticles Zebrafish embryos Single-cell RNA sequencing ERYTHROPOIESIS 

分 类 号：X52[环境科学与工程—环境工程] X171.5