慢性牙周炎患者病原菌及龈沟液RANKL/OPG、MMPs/TIMPs、SFRP1水平与病情程度的关系  

Relationship between pathogenic bacteria and RANKL/OPG,MMPs/TIMPs,SFRP1 levels in gingival crevicular fluid and disease severity of patients with chronic periodontitis

作　　者：苏林 张艳 刘恒林 时荣新 庄克川 SU Lin;ZHANG Yan;LIU Henglin;SHI Rongxin;ZHUANG Kechuan(Tengzhou Central People's Hospital affiliated to Xuzhou Medical University,Tengzhou,Shandong 277599,China)

机构地区：[1]徐州医科大学附属滕州市中心人民医院口腔科,山东滕州277599

出　　处：《中华医院感染学杂志》2025年第3期341-346,共6页Chinese Journal of Nosocomiology

基　　金：山东省中医药科技基金资助项目(Q-2022016);徐州医科大学优秀人才基金资助项目(XYFY202415)。

摘　　要：目的研究慢性牙周炎(CP)患者病原菌及龈沟液细胞核因子κB受体活化因子配体(RANKL)/骨保护素(OPG)、基质金属蛋白酶(MMPs)/基质金属蛋白酶抑制剂(TIMPs)、分泌型卷曲相关蛋白1(SFRP1)水平,并分析各指标与病情程度的关系。方法选取2021年1月—2023年12月滕州市中心人民医院收治的187例CP患者作为病例CP组,统计CP患者感染病原菌分布情况;选取同期医院体检的195名健康者作为牙周健康组,比较牙周健康情况及龈沟液RANKL/OPG、MMPs/TIMPs、SFRP1水平;将CP患者根据病情程度分为轻度CP组(93例)、中度CP组(69例)、重度CP组(25例),比较三组龈沟液RANKL/OPG、MMPs/TIMPs、SFRP1水平,采用Pearson相关系数分析CP患者龈沟液RANKL/OPG、MMPs/TIMPs、SFRP1水平与牙周健康的相关性。结果187例感染所致CP患者共分离病原菌195株,以牙龈卟啉单胞菌(26.15%)、福赛类杆菌(22.05%)为主。病例CP组牙周健康指标高于健康组,病例CP组龈沟液RANKL/OPG、基质金属蛋白酶-1(MMP-1)、MMP-3、MMP-8、MMP-9、SFRP1分别为(0.78±0.25)、(187.83±22.01)ng/ml、(25.98±3.17)ng/ml、(140.69±16.48)ng/ml、(115.37±14.27)ng/ml、(408.54±58.77)pg/ml均高于牙周健康组(P<0.05),龈沟液TIMP-1为(91.26±9.69)ng/ml低于牙周健康组(P<0.05)。轻度CP组、中度CP组、重度CP组龈沟液RANKL/OPG、MMP-1、MMP-3、MMP-8、MMP-9、SFRP1水平呈升高趋势,龈沟液TIMP-1水平呈降低趋势(P<0.05)。CP患者龈沟液RANKL/OPG、MMP-1、MMP-3、MMP-8、MMP-9、SFRP1与牙周健康指标呈正相关(P<0.05),龈沟液TIMP-1水平与牙周健康指标呈负相关(P<0.05)。结论CP患者主要感染病原菌种类包括牙龈卟啉单胞菌、福赛类杆菌,其牙周健康情况较差,且病情的发生和发展伴随着龈沟液RANKL/OPG、MMPs/TIMPs、SFRP1水平变化,同时龈沟液RANKL/OPG、MMPs/TIMPs、SFRP1水平与其牙周健康密切相关。OBJECTIVE To study the relationship between pathogenic bacteria and levels of nuclear factorκB receptor activator ligand(RANKL)/osteoprotegerin(OPG),matrix metalloproteinases(MMPs)/matrix metalloproteinases inhibitors(TIMPs)and secretory curl related protein 1(SFRP1)gingival crevicular fluid and severity disease in patient with chronic periodontitis(CP).METHODS Totally 187 cases of CP patients admitted to Tengzhou Central People's Hospital from Jan.2021 to Dec.2023 were selected as the case CP group,and the distribution of infectious pathogens in CP patients was counted.Another 195 healthy people who underwent physical examination during the same period were selected as the periodontal healthy group.The periodontal condotion and the levels of RANKL/OPG,MMPs/TIMPs and SFRP1 in gingival crevicular fluid were compared among the groups.Patients with CP were divided into the mild CP group(93 cases),the moderate CP group(69 cases)and the severe CP group(25 cases)according to the severity of the disease.The levels of RANKL/OPG,MMPs/TIMPs and SFRP1 in gingival crevicular fluid of the three groups were compared,and the correlation between the levels of RANKL/OPG,MMPs/TIMPs and SFRP1 in gingival crevicular fluid and periodontal condition was analyzed by Pearson correlation coefficient.RESULTS A total of 195 strains of pathogenic bacteria were isolated from 187 CP patients with infection,among which Porphyromonas gingivalis and Bacteroides Forsythiae accounted for 26.15%and 22.05%respectively.The levels of periodontal health in dicatonrs of the CP group were higher than these of the healthy group.The RANKL/OPG,matrix metalloproteinase-1(MMP-1),MMP-3,MMP-8,MMP-9 and SFRP1 in gingival crevicular fluid of the CP group were(0.78±0.25),(187.83±22.01)ng/ml,(25.98±3.17)ng/ml,(140.69±16.48)ng/ml,(115.37±14.27)ng/ml and(408.54±58.77)pg/ml respectively,all higher than those in the periodontal healthy group,while the TIMP-1 in the CP group was(91.26±9.69)ng/ml,lower than that in the periodontal healthy group(P<0.05).The levels

关 键 词：慢性牙周炎 病原菌 细胞核因子κB受体活化因子配体 骨保护素 基质金属蛋白酶 基质金属蛋白酶抑制剂 分泌型卷曲相关蛋白1 病情程度 相关性 

分 类 号：R781.42[医药卫生—口腔医学]