Caterpillar feeding drives patterns of induced defenses and herbivore resistance in wild Pinus tabuliformis  

作　　者：Tianhua Sun Guona Zhou Yanan Zhao Suhong Gao Jie Liu Junxia Liu Baojia Gao 

机构地区：[1]Hebei Key Laboratory for Forest Germplasm Resources and Forest Protection,Hebei Agricultural University Baoding,Hebei,071033,People’s Republic of China [2]College of Agronomy and Biotechnology,Hebei Normal University of Science and Technology,Changli,066612,People’s Republic of China

出　　处：《Journal of Forestry Research》2025年第1期391-411,共21页林业研究(英文版)

基　　金：supported by the Science and Technology Development Program of Hebei Agricultural University,the Research on Molecular Mechanisms of Population Differentiation and Adaptation of Forest Pests and Insects under Environmental Stress(grant No.:30771739);Forest Pests and Diseases(grant No.:1528003);the National Natural Science Foundation of China for the study of community regulatory mechanisms of insect pest pandemics in larch plantation forests(Grant No.:32371882).

摘　　要：The defense mechanisms induced in wild Chinese pine(Pinus tabuliformis)in response to herbivores are not well characterized,especially in the field.To address this knowledge gap,we established a biological model system to evaluate proteome variations in pine needles after feeding by the Chinese pine caterpillar(Dendrolimus tabulaeformis),a major natural enemy and dominant herbivore.Quantitative tandem mass tag(TMT)proteomics and bioinformatics were utilized to systematically identify differentially abundant proteins implicated in the induced defense response of Chinese pine.We validated key protein changes using parallel reaction monitoring(PRM)technology.Pathway analysis revealed that the induced defenses involved phenylpropanoid,coumarin,and flavonoid biosynthesis,among other processes.To elucidate the regulatory patterns underlying pine resistance,we determined the activities of defense enzymes and levels of physiological and biochemical compounds.In addition,the expression of upstream genes for key proteins was validated by qRT-PCR.Our results provide new molecular insights into the induced defense mechanisms in Chinese pine against this caterpillar in the field.A better understanding of these defense strategies will inform efforts to breed more-resistant pine varieties.

关 键 词：Pinus tabuliformis Dendrolimus tabulaeformis PROTEOMICS Induced Defensive responses 

分 类 号：O62[理学—有机化学]