不同光照时长对NIH小鼠体重和学习记忆能力的影响  

Effects of Different Durations of Light Exposure on Body Weight and Learning and Memory Abilities of NIH Mice

作　　者：张楠 李怀银 连晓娣 魏娟鹏 高明 ZHANG Nan;LI Huaiyin;LIAN Xiaodi;WEI Juanpeng;GAO Ming(Lanzhou Institute of Biological Products Co.,Ltd.,Lanzhou 730046,China)

机构地区：[1]兰州生物制品研究所有限责任公司,兰州730046

出　　处：《实验动物与比较医学》2025年第1期73-78,共6页Laboratory Animal and Comparative Medicine

摘　　要：目的研究不同光照时长对发育期NIH小鼠体重和学习记忆能力的影响。方法实验选取体重相近的发育期NIH小鼠40只,雌雄各半。小鼠经每天12 h光照时长适应性饲养1周后,被随机分为每天0、6、12、18、24 h光照时长组,每组8只,实验周期为7周,其中前5周为不同光照时长条件下的饲养阶段,后2周为行为学实验阶段。通过体重监测以及T型迷宫、新位置识别和八臂迷宫等行为学实验,分析光照时长对小鼠体重和学习记忆能力的影响。结果在光照处理期间,各组小鼠间的体重变化无显著差异(P>0.05);在光照处理第2周和第3周,每天24 h光照时长组小鼠的体重增长量显著高于每天0 h和6 h光照时长组(P<0.05)。在光照处理5周后,T型迷宫实验中,每天0 h光照时长组小鼠的潜伏期时间极显著长于每天12 h光照时长组(P<0.01);每天24 h光照时长组小鼠的潜伏期时间显著长于每天12 h光照时长组(P<0.05)。新位置识别实验中,每天12 h光照时长组小鼠的辨别指数和新位置观察时间均长于其他组,且与每天18 h光照时长组差异极显著(P<0.01),与每天24 h光照时长组差异显著(P<0.05);八臂迷宫实验中,每天12 h光照时长组小鼠找到饲料的时间、参考记忆错误比率和工作记忆错误比率均短于每天0 h光照时长组,且差异显著(P<0.05);而每天24 h光照时长组小鼠的工作记忆错误比率高于每天12 h光照时长组,且差异显著(P<0.05)。结论每天24 h光照会影响NIH小鼠的体重增长,而每天光照时长超过18 h或低于6 h会减弱NIH小鼠的学习记忆能力。Objective This study aims to investigate the effects of varying durations of light exposure on body weight and learning and memory abilities of pubertal NIH mice.Methods Forty pubertal NIH mice,evenly split by gender and with similar initial weights,were subjected to a 12 h light-dark cycle for one week.They were then randomly assigned to groups with daily light exposure durations of 0,6,12,18,and 24 hours,with 8 mice in each group.The experimental period lasted for 7 weeks,with the first 5 weeks as the feeding phase under different light exposure conditions,and the last 2 weeks as the behavioral testing phase.Their body weight was monitored,and learning and memory abilities were assessed using the T-maze,object location test,and eight-arm maze tests.Results During the light exposure period,there were no significant differences in body weight among groups(P>0.05).However,the weight gain of mice in the 24 h group was significantly higher than that of the 0 h group and the 6 h group during the second and third weeks of light exposure(P<0.05).After five weeks of light exposure,in the T-maze test,the latency time of the 0 h light exposure group was significantly longer than that of the 12 h group(P<0.01),and the latency time of the 24 h light exposure group was significantly longer than that of the 12 h group(P<0.05).In the object location test,the mice in 12 h group exhibited a higher discrimination index and spent more time observing the new location compared to the other groups,with significant differences in comparison to the 18 h group(P<0.01)and the 24 h group(P<0.05).In the eight-arm maze test,the time to find food,the reference memory error rate,and the working memory error rate in the 12 h group were all lower than those in the 0 h group,with significant differences(P<0.05).Moreover,the working memory error rate in the 24 h group was higher than that in the 12 h group,with significant differences(P<0.05).Conclusion Continuous 24 h light exposure affects body weight gain,while light exposure durations exceedi

关 键 词：NIH小鼠 光照时长 体重 学习记忆能力 

分 类 号：Q95-33[生物学—动物学] R-332[医药卫生]