LncRNA PCAT-1对鼻咽癌细胞生物学行为及化疗敏感性的影响  

Effect of LncRNA PCAT-1 on the biological behaviour and chemosensitivity of nasopharyngeal carcinoma cells

作　　者：张茂华 魏日富 朱忠寿 刘平 高尚 李慧凤[1] ZHANG Maohua;WEI Rifu;ZHU Zhongshou;LIU Ping;GAO Shang;LI Huifeng(Department of Otorhinolaryngology&Head and Neck Surgery,Ningde Affiliated Hospital of Ningde Normal University,Ningde 352100,Fujian,China;Department of Otorhinolaryngology&Head and Neck Surgery,Shanghai General Hospital,Shanghai 200080,China)

机构地区：[1]宁德师范学院附属宁德市医院耳鼻咽喉头颈外科,福建宁德352100 [2]上海市第一人民医院耳鼻咽喉头颈外科,上海200080

出　　处：《山东大学耳鼻喉眼学报》2025年第1期68-76,共9页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：福建省自然科学基金项目(2021J011159)。

摘　　要：目的探讨长链非编码RNA转录产物1(long non-coding RNA Prostate cancer associated transcript-1,lncRNA PCAT-1)对鼻咽癌HK-1细胞生物学行为及化疗敏感性的影响。方法实时定量PCR(Real-time quantitative PCR,RT-qPCR)检测LncRNA PCAT-1在不同鼻咽癌细胞株中的表达,CCK8、transwell、流式细胞术检测过表达/干扰LncRNA PCAT-1的HK-1细胞增殖、迁移、侵袭、凋亡的变化,蛋白免疫印记(Western blot,WB)检测上皮间质转化(epithelial-mesenchymal transition,EMT)通路相关蛋白的表达,细胞增殖实验检测5-FU及DDP的半数抑制率(median inhibition concentration,IC50)变化。结果LncRNA PCAT-1在HK-1细胞株中表达升高。过表达LncRNA PCAT-1后,HK-1细胞的增殖、迁移、侵袭显著增加,而凋亡减少;E-cadherin蛋白表达水平显著降低,vimentin和N-cadherin蛋白表达水平显著升高;过表达后HK-1细胞对5-FU及DDP的IC50升高,细胞克隆数增加。干扰LncRNA PCAT-1后则相反,HK-1细胞增殖、迁移、侵袭显著降低,而凋亡增加;E-cadherin蛋白表达量显著升高,vimentin和N-cadherin蛋白表达水平显著降低;干扰后HK-1细胞对5-FU及DDP的IC50降低,细胞克隆数减少;差异均有显著性意义(P<0.05)。结论lncRNA PCAT-1通过EMT途径来调控鼻咽癌HK-1细胞的生物学行为,并降低细胞对5-FU和DDP的药物敏感性。Objective To investigate the effect of(long non-coding RNA Prostate cancer associated transcript-1,lncRNA PCAT-1)on the biological behaviour and chemosensitivity of nasopharyngeal carcinoma HK-1 cells.Methods Real-time quantitative PCR(RT-qPCR)was used to detect the expression of LncRNA PCAT-1 in different nasopharyngeal carcinoma cell lines.CCK8,Transwell,and flow cytometry were used to detect changes in cell proliferation,migration,invasion,and apoptosis of HK-1 cells overexpressing/interfering with LncRNA PCAT-1.Western blot(WB)was used to detect the expression of proteins associated with the epithelial-mesenchymal transition(EMT)pathway.Cell proliferation experiments were used to detect changes in the median inhibition concentration(IC50)of 5-FU and DDP.Results The expression of LncRNA PCAT-1 was increased in nasopharyngeal carcinoma cell lines.Overexpression of LncRNA PCAT-1 significantly increased the proliferation,migration,and invasion of HK-1 cells,while decreasing apoptosis;the expression level of E-cadherin protein significantly decreased,while the expression levels of vimentin and N-cadherin proteins were significantly increased.Overexpression also increased the IC50 of HK-1 cells against 5-FU and DDP and the number of cell clones.Interference with LncRNA PCAT-1,on the contrary,led to reduced proliferation,migration,and invasion,with increased apoptosis in HK-1 cells.The expression level of E-cadherin protein significantly increased,while the expression levels of vimentin and N-cadherin proteins significantly decreased.After interference,the IC50 of HK-1 cells against 5-FU and DDP decreased,and the number of cell clones reduced.All differences were statistically significant(P<0.05).Conclusion LncRNA PCAT-1 regulates the biological behavior of HK-1 nasopharyngeal carcinoma cells through the EMT pathway and reduces their drug sensitivity to 5-FU and DDP.

关 键 词：鼻咽癌细胞 长链非编码RNA转录产物1 上皮间质转化 化疗敏感性 

分 类 号：R739.6[医药卫生—肿瘤] R766.3[医药卫生—临床医学]