COL5A1基因在肿瘤预后和免疫浸润中作用的泛癌分析  

Pan-Cancer Analysis of COL5A1 Expression and Its Biological and Clinical Implication

作　　者：葛茹茹 方潇 高帅 戴天增 周智华 GE Ruru;FANG Xiao;GAO Shuai;DAI Tianzeng;ZHOU Zhihua(MengChao Hepatobiliary Hospital of Fujian Medical University,Fuzhou 350025,China)

机构地区：[1]福建医科大学孟超肝胆医院,福建福州350025

出　　处：《肿瘤学杂志》2025年第1期51-58,共8页Journal of Chinese Oncology

基　　金：福建省自然科学基金(2020J011172)。

摘　　要：[目的]分析泛癌中的COL5A1基因及蛋白表达水平,并探讨其与临床预后和肿瘤免疫浸润的关系。[方法]分析癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中肿瘤患者的数据,采用TIMER2.0、GEPIA2、SangerBox、人类蛋白图谱(human protein atlas,HPA)和STRING分析等生物信息学工具分析COL5A1基因与泛癌的关系。[结果] COL5A1在17种不同肿瘤和癌旁组织中的表达水平有差异。COL5A1高表达与膀胱尿路上皮癌、肺鳞状细胞癌、肺腺癌、宫颈鳞状细胞癌和宫颈腺癌、甲状腺癌、卵巢癌、肾透明细胞癌、肾乳头状细胞癌和胃腺癌总生存期相关(P均<0.05)。COL5A1表达与肿瘤微环境免疫检查点、肿瘤突变负荷和微卫星不稳定性显著性相关。COL5A1表达与B细胞、T细胞、中性粒细胞、巨噬细胞和抗原递呈细胞相关。[结论] COL5A1是肿瘤微环境的重要组成部分,影响免疫细胞浸润,可作为评估多种恶性肿瘤的免疫浸润和预后分析的肿瘤标志物。[Objective]To investigate the gene and protein expression levels of the COL5A1 in pan-cancer and its biological and clinical implication.[Methods]Data from cancer patients were collected from The Cancer Genome Atlas(TCGA)database,and its biological and clinical implication in pan-cancer were analyzed with TIMER2.0,GEPIA2,SangerBox,human protein atlas(HPA)and STRING.[Results]The gene and protein expression levels of COL5A1 varied in 17 different tumors and adjacent tissues.High expression of COL5A1 was significantly associated with shorter overall sur-vival in bladder urothelial carcinoma,lung squamous cell carcinoma,lung adenocarcinoma,cer-vical squamous cell carcinoma and cervical adenocarcinoma,thyroid cancer,ovarian cancer,clear cell renal carcinoma,papillary renal carcinoma,and gastric adenocarcinoma(all P<0.05).COL5A1 expression was significantly correlated with immune checkpoints in the tumor microenvi-ronment,tumor mutation burden,and microsatellite instability in human cancers.Positive expres-sion of COL5A1 was associated with immune infiltration of B cells,T cells,neutrophils,macrophages,and antigen-presenting cells in tumors.[Conclusion]COL5A1 is an important com-ponent of the tumor microenvironment,influencing immune cell infiltration,and it can serve as a tumor marker for assessing immune infiltration and prognosis in various malignant tumors.

关 键 词：COL5A1 肿瘤微环境 预后 免疫浸润 泛癌分析 

分 类 号：R730.231[医药卫生—肿瘤]