基于双硫死亡相关基因构建心力衰竭临床诊断模型的价值  

Value of a clinical diagnostic model of heart failure based on disulfidptosis-related genes

作　　者：李省 陈霞[1] 杨沛垚 郭艳丽 王丽[1] 马克涛[2] Li Sheng;Chen Xia;Yang Peiyao;Guo Yanli;Wang Li;Ma Ketao(Heart Center,the First Affiliated Hospital of Shihezi University,Shihezi 832008,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]石河子大学第一附属医院心脏中心,832008 [2]石河子大学医学院生理学教研室

出　　处：《中华老年心脑血管病杂志》2025年第3期370-373,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：兵团指导性科技计划项目(2023ZD001);兵团财政科技计划项目(2020AB023)。

摘　　要：目的探究基于双硫死亡相关基因构建心力衰竭临床诊断模型的价值。方法获取训练集基因表达谱系列号(Gene Expression Omnibus Series,GSE)57345的差异表达双硫死亡相关基因,进行基因本体论(Gene Ontology,GO)、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析、Metascape疾病富集分析。C57BL/6J雄性小鼠6只随机分为对照组和心力衰竭(heart failure,HF)组,每组3只,对照组给予生理盐水腹腔注射,HF组腹腔注射异丙肾上腺素。使用实时荧光定量聚合酶链反应检测关键基因的表达水平。结果GO富集分析主要涉及血小板聚集等方面;KEGG显著富集在紧密接头、血管平滑肌收缩等信号通路。Metascape富集分析显示,差异表达基因主要与局灶性肾小球硬化症、肾小球疾病、血小板疾病、肿瘤浸润、肾病综合征等疾病有关。HF组小鼠心体比表达明显高于对照组,心脏射血分数、短轴缩短率、心排血量、每搏量明显低于对照组,差异有统计学意义(P<0.05,P<0.01)。HF组小鼠心脏组织蛋白BNP、基因MYH10 mRNA表达明显高于对照组[1.026±0.501 vs 0.686±0.187,P=0.038;1.469(1.782,2.670)vs 0.360(0.786,1.117),P=0.000],心脏组织基因MYL6、TLN1 mRNA表达明显低于对照组,差异有统计学意义(0.575±0.105 vs 1.000±0.202,P=0.027;0.429±0.114 vs 1.000±0.109,P=0.000)。结论构建的心力衰竭诊断模型具有较好的诊断性能。Objective To explore the value of a clinical diagnostic model of heart failure(HF)based on disulfidptosis-related genes.Methods The differentially expressed disulfidetosis-related genes from the training set of Gene Expression Omnibus Series(GSE)57345 were obtained,and then analyzed with Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis,and Metascape disease enrichment analysis.Six male C57BL/6J mice were randomly divided into control group(intraperitoneal injection of normal saline)and HF group(intraperitoneal injection of isoproterenol),with 3 mice in each group.Real-time quantitative PCR was applied to detect the expression levels of key genes.Results GO enrichment analysis revealed that the differentially expressed disulfidetosis-related genes were mainly involved in platelet aggregation and other aspects.KEGG showed they were significantly enriched in tight junctions,vascular smooth muscle contraction and other signaling pathways.Metascape enrichment analysis indicated that these genes were mainly related to focal glomerulosclerosis,glomerular disease,platelet disease,tumor infiltration,nephrotic syndrome and other diseases.The HF group had significantly higher heart weight-to-body weight ratio,and lower ejection fraction,fractional shortening,cardiac output and stroke volume than the control group(P<0.05,P<0.01).The cardiac mRNA levels of BNP and MYH10 were significantly higher[1.026±0.501 us 0.686±0.187,P=0.038;1.469(1.782,2.670)vs 0.360(0.786,1.117),P=0.000],while those of MYL6 and TLN1 was obviously lower(0.575±0.105 us 1.000±0.202,P=0.027;0.429±0.114 s 1.000±0.109,P=0.000)in the HF group than the control group.Conclusion Our constructed HF diagnostic model has better diagnostic performance.

关 键 词：心力衰竭 计算生物学 免疫 细胞 基因表达 

分 类 号：R541.6[医药卫生—心血管疾病]