红景天苷联合肌外膜管及雪旺细胞促进大鼠坐骨神经损伤延期修复神经功能改善的实验研究  

Salidroside combined with epimysium conduit and Schwann cells promotes neurologic function recovery after delayed repair of sciatic nerve injury in rats

作　　者：漆景天 叶永平 徐永君 盛青松 张永光 Qi Jing-tian;Ye Yong-ping;Xu Yong-jun;Sheng Qing-song;Zhang Yong-guang(Department of Orthopaedics,Fuzong Clinical Medicine College of Fujian Medical University,Fuzhou,Fujian 350025,China;Fujian Provincial Key Laboratory of Transplantation Biology,Fuzong Clinical Medicine College of Fujian Medical University,Fuzhou,Fujian 350025,China;Department of Obstetrics and Gynecology,Fuzong Clinical Medicine College of Fujian Medical University,Fuzhou,Fujian 350025,China)

机构地区：[1]福建医科大学福总临床医学院骨科,福建福州350025 [2]福建医科大学福总临床医学院移植生物学重点实验室,福建福州350025 [3]福建医科大学福总临床医学院妇产科,福建福州350025

出　　处：《中国现代医学杂志》2025年第6期32-38,共7页China Journal of Modern Medicine

基　　金：福建省自然科学基金(No:2021J011273,No:2022J01492);第九〇〇医院院内课题(No:2023ZS07)。

摘　　要：目的探讨红景天苷联合肌外膜管及雪旺细胞对坐骨神经损伤大鼠延迟修复后神经再生的潜在作用。方法48只SD大鼠随机分为肌外膜管组(EPM)、肌外膜管+RSC96雪旺细胞组(EPM+RSC96)、0.2 mmol/L红景天苷组(L-ros)、0.4 mmol/L红景天苷组(H-ros),复制大鼠坐骨神经损伤延迟修复模型。其中EPM组只使用肌外膜管桥接神经缺损,EPM+RSC96组肌外膜管内注入RSC96细胞和BD基质凝胶,L-ros组、H-ros组分别注入0.2、0.4 mmol/L红景天苷与RSC96细胞和BD基质凝胶的混合物。术后12周,通过透射电镜评估再生神经的形态。术后8、12周,通过坐骨神经功能指数(SFI)、腓肠肌湿重及Masson染色分析评估运动功能的恢复。结果EPM+RSC96组、L-ros组、H-ros组再生轴突直径、再生髓鞘厚度均高于EPM组(P<0.05),L-ros组高于EPM+RSC96组(P<0.05),H-ros组高于L-ros组(P<0.05)。EPM+RSC96组术后8、12周SFI高于EPM组(P<0.05),L-ros组高于EPM+RSC96组(P<0.05),H-ros组术后12周高于L-ros组(P<0.05)。L-ros组、H-ros组术后8和12周腓肠肌湿重恢复率均高于EPM组(P<0.05),H-ros组高于EPM+RSC96组、L-ros组(P<0.05)。L-ros组、H-ros组术后8和12周腓肠肌肌纤维百分比均高于EPM组、EPM+RSC96组(P<0.05)。结论红景天苷联合肌外膜管及雪旺细胞对坐骨神经损伤模型大鼠延迟修复后的神经功能恢复具有促进作用。Objective To investigate the potential effects of salidroside combined with epimysium conduit and Schwann cells on nerve regeneration after delayed repair of sciatic nerve injury in rats.Methods Forty-eight SD rats were randomly divided into the epimysium conduit(EPM)group,the epimysium conduit+RSC96 Schwann cell(EPM+RSC96)group,the 0.2 mmol/L salidroside(L-ros)group,and the 0.4 mmol/L Salidroside(H-ros)group,and a delayed repair model of sciatic nerve injury in rats was established.The EPM group used only the epimysium conduit to bridge the nerve defects,the RSC96 cells and the BD matrix gel were injected into the epimysium conduit in the EPM+RSC96 group,and a mixture of 0.2 mmol/L or 0.4 mmol/L salidroside with RSC96 cells and BD matrix gel was injected into the epimysium conduit in the L-ros and the H-ros groups,respectively.The morphology of the regenerated nerves was assessed by transmission electron microscopy at 12 weeks after surgery.Recovery of motor function was evaluated by sciatic nerve function index(SFI),wet muscle weight(WMW)of the bilateral gastrocnemius muscles,and Masson staining at 8 and 12 weeks after surgery.Results The diameter of regenerated axons and the thickness of regenerated myelin sheath in the EPM+RSC96 group,L-ros group,and H-ros group were all greater than those in the EPM group(P<0.05),those in the L-ros group were greater than in the EPM+RSC96 group(P<0.05),and those in the H-ros group were even greater than those in the L-ros group(P<0.05).The SFI at 8 and 12 weeks after surgery in the EPM+RSC96 group was higher than that in the EPM group(P<0.05),and that in the L-ros group was higher than that in the EPM+RSC96 group(P<0.05).At 12 weeks postoperatively,the SFI in the H-ros group was higher than that in the L-ros group(P<0.05).The recovery rates of WMW of gastrocnemius muscles at 8 and 12 weeks after surgery were higher in the L-ros group and the H-ros group than in the EPM group(P<0.05),while those in the H-ros group were higher than those in the EPM+RSC96 group and the L-ros gr

关 键 词：坐骨神经损伤 红景天苷 肌外膜管 延迟修复 神经再生 

分 类 号：R745.42[医药卫生—神经病学与精神病学]