年轻家兔髁突吸收动物模型的建立与研究  

Establishment and research of an animal model for condylar resorption in young domestic rabbits

作　　者：金雪梅 安玮[1,2] 郭莎 阿拉发提·何亚斯丁 加娜尔·吐根别克 姚志涛 Jin Xuemei;An Wei;Guo Sha;Alahati He Yasding;Ganar Turgenbuk;Yao Zhitao(Department of Oral and Maxillofacial Trauma and Orthog Nathic Surgery,First Affiliated Hospital of Xinjiang Medical University(Affiliated School of Stomatology),Urumqi 830054,China;Xinjiang Uygur Autonomous Region Institute of Stomatology,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院(附属口腔医学院)口腔颌面创伤正颌外科,乌鲁木齐830054 [2]新疆维吾尔自治区口腔医学研究所,乌鲁木齐830054

出　　处：《中华口腔医学研究杂志(电子版)》2025年第1期16-24,共9页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：新疆维吾尔自治区“天山英才”培养计划(2023TSYCCX0055);新疆维吾尔自治区研究生创新项目(XJ2024G177)。

摘　　要：目的探讨Ⅲ类杠杆生物力学原理对构建年轻家兔髁突吸收模型的影响。方法以16只6月龄雌性家兔为研究对象,随机数字表法分为2周实验组、4周实验组、2周对照组及4周对照组,每组4只。2个实验组进行髁突吸收造模处理,2个对照组不做任何处理。髁突吸收造模方法:于家兔下颌前牙堆塑强度较高的树脂突,用此阻止上下颌牙在咀嚼运动时的正常咬合,使家兔被动做下颌后退动作咀嚼磨碎食物,使双侧颞下颌关节(TMJ)在Ⅲ类杠杆生物力的长期压迫作用下出现髁突吸收。2周实验组与2周对照组于建模2周时处死取材,4周实验组与4周对照组于建模4周时处死取材。通过对家兔TMJ部位CT三维重建观察并测量髁突表面积及体积变化,对髁突组织进行苏木精-伊红染色及外周血进行Elisa检测测量骨代谢水平。使用SPSS 19.0统计软件对各实验组与对照组数据进行独立样本t检验。结果(1)CT三维重建:2周实验组髁突体积[(196±8)mm^(3)]、表面积[(280±12)mm^(2)]较对照组体积[(204±9)mm^(3)]和表面积[(292±13)mm^(2)]有所减小,差异有统计学意义(t体积=4.409,P体积=0.022;t表面积=3.660,P表面积=0.035)。4周实验组髁突体积[(192±18)mm^(3)]、表面积[(280±10)mm^(2)]较对照组体积[(211±11)mm^(3)]、表面积[(309±10)mm^(2)]减小,差异有统计学意义(t_(体积)=4.081,P_(体积)=0.027;t_(表面积)=3.738,P_(表面积)=0.033)。同一建模周期,左右侧髁突表面积与体积变化程度未见明显差异。(2)苏木精-伊红染色:2周实验组髁突增殖层细胞增多,细胞簇状聚集,出现均质样无细胞区域。4周实验组髁突软骨分层不明显,肥大层细胞及核明显增大,骨小梁间隙中充满大量的红细胞。(3)关节盘肉眼观:2周实验组及4周实验组关节盘中间带较对照组透光程度增加。(4)骨代谢指标:血清骨钙素浓度2周实验组[(35.8±1.4)ng/mL]较2周对照组[(18.1±8.1)ng/mL]增高,差异有统�Objective Exploring the effect of class Ⅲ lever biomechanical principles on the construction of a condylar resorption model in young rabbits.Methods A total of sixteen 6-month-old female rabbits were randomizedly divided into four groups,i.e.,2-week experimental group,4-week experimental group,2-week control group,and 4-week control group,with 4 rabbits in each group.The experimental groups were treated with condylar resorption modeling,and the control groups were left untreated.The condylar resorption modeling method was as follows.The mandibular anterior teeth of rabbits were stacked with high-strength resin protrusions,which prevented the normal occlusion of the maxillary and mandibular teeth during mastication,so that the rabbits could passively perform mandibular backward movements to chew and grind food,and condylar resorption of bilateral temporomandibular joints occurred under the long-term pressure of the classⅢlever biological force.The 2-week experimental group and the 2-week control group were necropsied after 2 weeks of modeling,whereas the 4-week experimental group and the 4-week control group were necropsied after 4 weeks of modeling.Bone metabolism was measured by CT scanning and 3D reconstruction of the temporomandibular joints of rabbits.Specifically,condylar surface area and volume changes,hematoxylin-eosin staining of condylar tissues,and Elisa assay of peripheral blood were evaluated.Independent samples t-test was used for statistical analysis of the data with software(SPSS 19.0).Results(1)CT 3D reconstruction:The volume of the condyle[(196±8)mm^(3)]and the surface area[(280±12)mm^(2)]in the 2-week experimental group significantly decreased compared to those of the control group[(204±9)mm^(3),(292±13)mm^(2);t_(volume)=4.409,P_(volume)=0.022;t_(surfacearea)=3.660,P_(surfacearea)=0.035].The volume of the condyle[(192±18)mm^(3)]and the surface area[(280±10)mm^(2)]in the 4-week experimental group significantly decreased compared to those of the control group[(211±11)mm^(3),(309±10)mm^

关 键 词：年轻家兔 髁突吸收 下颌后缩 Ⅲ类生物杠杆力 动物模型 

分 类 号：R782[医药卫生—口腔医学]