番茄GGP基因在抗坏血酸合成代谢中的功能鉴定  

Function Identification of GGP Gene in Ascorbic Acid Synthesis and Metabolism in Tomato

作　　者：李艳 赵红星 王勇 姜俊 孙联合 姬宇飞 高艳娜 李营 张星雨 张余洋[3] Li Yan;Zhao Hongxing;Wang Yong;Jiang Jun;Sun Lianhe;Ji Yufei;Gao Yanna;Li Ying;Zhang Xingyu;Zhang Yuyang(Zhumadian Agricultural Academy of Science,Zhumadian,46300;College of Horticulture,Henan Agricultural University,Zhengzhou,450002;Key Laboratory of Horticultural Plant Biology(Ministry of Education),Huazhong Agricultural University,Wuhan,430070)

机构地区：[1]驻马店市农业科学院,驻马店463000 [2]河南农业大学园艺学院,郑州450002 [3]华中农业大学,园艺植物生物学教育部重点实验室,武汉430070

出　　处：《分子植物育种》2025年第1期42-47,共6页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2018YFD1000800);国家自然科学基金项目(31991182,31972426);国家大宗蔬菜产业技术体系项目(CARS-23-G-18);武汉市应用基础前沿项目(2019020701011492)共同资助。

摘　　要：番茄(Solanum lycopersicum)抗坏血酸合成代谢基因GGP等相继被克隆,但是其在番茄抗坏血酸合成代谢中的作用还有待深入研究。本研究构建了番茄GGP基因超量和干涉(RNAi)表达载体,通过农杆菌介导的遗传转化方法将其转入番茄品系Ailsa Craig(AC)中,并对转基因材料进行GGP基因及合成途径关键基因表达量及相应抗坏血酸含量的测定。结果表明,超量表达番茄自身GGP基因后,株系O4-3和O13-9破色期果实总抗坏血酸含量相比对照分别提高了11.08%和4.03%;番茄GGP基因干涉抑制后,株系R2-2和R9-6中果实总抗坏血酸含量相比对照材料显著下降,分别下降了71.19%和81.22%。不同物种GGP进化分析表明,番茄GGP基因与茄科作物中GGP基因亲缘关系较近,其中番茄与马铃薯亲缘关系最近。本研究初步探索了GGP基因在番茄抗坏血酸合成代谢调控中的作用。In tomato(Solanum lycopersicum),genes involved in ascorbic acid metabolism likes GGP were successively identified.The roles of these genes in ascorbic acid synthesis and metabolism in tomato await further investigation.The overexpression and RNA interference(RNAi)vector of tomato GGP was constructed and transformed into genome of tomato variety Ailsa Craig using the Agrobacterium-mediated transformation,and the expression levels of GGP gene and key genes of synthesis pathway and corresponding ascorbic acid content of transgenic materials were measured.The results indicated that the total ascorbic acid content in breaker fruits of overexpression lines,O4-3 and O13-9,increased by 11.08%and 4.03%respectively relative to the control,whereas the total ascorbic acid content in breaker fruits of RNAi lines,R2-2 and R9-6,decreased significantly by 71.19%and81.22%relative to the control.The analysis of GGPs evolution in different species showed that the GGPs gene in tomato was closely related to the GGPs gene in Solanaceae crops,and the GGP gene in tomato and potato was the most closely related.This study preliminarily analyzed the function of GGP in regulating tomato ascorbic acid biosynthesis and metabolism.

关 键 词：番茄 抗坏血酸 GGP 遗传转化 

分 类 号：S641.2[农业科学—蔬菜学]