鼻咽癌组织中lncRNA ANCR表达与临床病理特征的相关性研究  

Correlation of lncRNA ANCR expression with clinicopathological characteristics in nasopharyngeal carcinoma tissues

作　　者：高妍[1] 王忠巧 GAO Yan;WANG Zhongqiao(Department of Otolaryngology,the Second Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang,830028)

机构地区：[1]新疆医科大学第二附属医院耳鼻喉科,新疆乌鲁木齐830028

出　　处：《实用临床医药杂志》2025年第3期1-5,共5页Journal of Clinical Medicine in Practice

基　　金：新疆维吾尔自治区自然科学基金资助项目(2022D01C504)。

摘　　要：目的探讨鼻咽癌组织中长链非编码RNA抗分化非编码RNA(lncRNA ANCR)表达与临床病理特征的相关性。方法随机纳入2015年6月—2019年6月在本院病理确诊鼻咽癌的115例患者为研究对象,采用实时荧光定量PCR法检测肿瘤组织与癌旁正常组织中lncRNA ANCR表达量。分析lncRNA ANCR表达与性别、年龄、病理类型、最大直径、分化级别、临床分期、淋巴结转移的相关性。随访60个月,记录患者肿瘤进展和总生存情况。结果肿瘤组织中lncRNA ANCR表达量高于癌旁正常组织,差异有统计学意义(P<0.001)。最大直径≥3 cm、低分化、Ⅲ~Ⅳ期、淋巴结转移阳性患者肿瘤组织中lncRNA ANCR表达量高于最大直径<3 cm、中高分化、Ⅰ~Ⅱ期、淋巴结转移阴性患者,差异有统计学意义(P<0.001)。肿瘤进展、死亡患者肿瘤组织中lncRNA ANCR表达量高于肿瘤未进展、存活患者,差异有统计学意义(P<0.001)。多因素Logistic回归分析显示,最大直径≥3 cm、Ⅲ~Ⅳ期、淋巴结转移阳性、lncRNA ANCR表达量与肿瘤进展密切相关,Ⅲ~Ⅳ期和lncRNA ANCR表达量与死亡密切相关(P<0.001)。受试者工作特征(ROC)曲线显示,肿瘤组织中lncRNA ANCR表达量预测肿瘤进展和死亡的曲线下面积(AUC)分别为0.899和0.842(P<0.001)。Kaplan-Meier生存曲线显示,肿瘤组织中lncRNA ANCR高表达组5年生存率低于lncRNA ANCR低表达组,差异有统计学意义(P<0.01)。结论肿瘤组织中lncRNA ANCR高表达可能参与鼻咽癌的恶性发展,与临床预后密切相关。Objective To investigate the correlation between the expression of long non-coding RNA anti-differentiation non-coding RNA(lncRNA ANCR)and clinicopathological characteristics in nasopharyngeal carcinoma tissues.Methods A total of 115 patients pathologically diagnosed as nasopharyngeal carcinoma in the hospital from June 2015 to June 2019 were randomly included as research objects,and real-time fluorescent quantitative PCR was used to detect the expression level of lncRNA ANCR in tumor tissues and adjacent normal tissues.Correlations of lncRNA ANCR expression with gender,age,pathological type,maximum diameter,differentiation grade,clinical stage,and lymph node metastasis were analyzed.Patients were followed up for 60 months to record tumor progression and overall survival.Results The expression level of lncRNA ANCR in tumor tissues was significantly higher than that in adjacent normal tissues(P<0.001).Patients with a maximum diameter≥3 cm,low differentiation,stageⅢtoⅣ,and positive lymph node metastasis had significantly higher lncRNA ANCR expression level in tumor tissues than those with a maximum diameter<3 cm,moderate-to-high differentiation,stageⅠtoⅡ,and negative lymph node metastasis(P<0.001).Patients with tumor progression and death outcome had significantly higher lncRNA ANCR expression level in tumor tissues than those without tumor progression and survival outcome(P<0.001).Multivariate Logistic regression analysis showed that a maximum diameter≥3 cm,stageⅢtoⅣ,positive lymph node metastasis,and lncRNA ANCR expression were significantly related to tumor progression,while stageⅢtoⅣand lncRNA ANCR expression were significantly related to death(P<0.001).The receiver operating characteristic(ROC)curve showed that the areas under the curve(AUC)for predicting tumor progression and death based on lncRNA ANCR expression level in tumor tissues were 0.899 and 0.842,respectively(P<0.001).The Kaplan-Meier survival curve showed that the 5-year survival rate in the high lncRNA ANCR expression group was

关 键 词：鼻咽癌 长链非编码RNA 抗分化非编码RNA 恶性肿瘤 病理特征 

分 类 号：R739.6[医药卫生—肿瘤] Q522[医药卫生—临床医学] R319[生物学—生物化学]