CmNLP7 interacts with CmPP6 to suppress flowering time in Chrysanthemum  

作　　者：Guohui Wang Juanjuan Wang Mengru Yin Yu Zhang Jiaxin Zhang Chaona Si Sumei Chen Zhiyong Guan Shuang Zhao Fei Zhang Weiming Fang Fadi Chen Jiafu Jiang 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization,Key Laboratory of Landscaping,Ministry of Agriculture and Rural Affairs,Key Laboratory of State Forestry and Grassland Administration on Biology of Ornamental Plants in East China,College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China [2]Zhongshan Biological Breeding Laboratory,No.50 Zhongling Street,Nanjing,Jiangsu 210014,China

出　　处：《Horticultural Plant Journal》2025年第2期865-876,共12页园艺学报(英文版)

基　　金：supported by grants from the National Natural Science Foundation of China(Grant No.31930100);a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions。

摘　　要：Nitrogen(N)is a key component in plants and their biological macromolecules,having a profound effect on developmental stages,such as germination,vegetative growth,and flowering.However,the mechanism of nitrogen-regulated flowering time remains unclear.In this study,CmNLP7 was isolated from the chrysanthemum cultivar‘Jinba'and was characterized.CmNLP7 is a transcription factor localized in the nucleus but has no transcriptional activity.Tissue expression pattern analysis showed that CmNLP7 was mainly transcribed in leaves and roots.Knocking down CmNLP7 through the artificial-miRNA method in chrysanthemum resulted in early flowering under optimal nitrogen(ON)and low nitrogen(LN)conditions;whereas overexpression lines showed delayed flowering under LN conditions.Transcriptome sequencing analysis showed that the nitrate transporters NRT2.5,NPF3.1,and NPF4.6;SBP-like genes SPL7 and SPL12,and flowering integration factor FT were significantly up-regulated in the knockdown lines.Based on the KEGG pathway enrichment analysis,the differentially transcribed genes were enriched in phenylpropanoid biosynthesis and starch and sucrose metabolism pathways,which indicated their alleged function in nitrogen-regulated flowering and development in chrysanthemum.Furthermore CmPP6 as a homolog of the Arabidopsis phosphatase PP6,was verified as an interacting protein of CmNLP7 by yeast two-hybrid,BiFC,pull-down and Biacore in vitro and in vivo,and the knockdown line of CmPP6(amiR-CmPP6)flowered earlier compared to that of the wild-type chrysanthemum‘Jinba'.Collectively,these results demonstrated that CmPP6 interacts with CmNLP7 to regulate chrysanthemum flowering,and CmNLP7 could regulate flowering time in response to nitrogen,which lays a foundation for the regulation of flowering and molecular breeding of chrysanthemum through changes in nutrient signaling.

关 键 词：Nitrogen FLOWERING CHRYSANTHEMUM CmNLP7 CmPP6 RNA-SEQ SPL 

分 类 号：S682.11[农业科学—观赏园艺]