Optical sectioning methods in three-dimensional bioimaging  

作　　者：Jing Zhang Wei Qiao Rui Jin Hongjin Li Hui Gong Shih-Chi Chen Qingming Luo Jing Yuan 

机构地区：[1]Britton Chance Center for Biomedical Photonics,Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan,China [2]MoE Key Laboratory for Biomedical Photonics,Innovation Institute,Huazhong University of Science and Technology,Wuhan,China [3]Hong Kong Center for Cerebro-Cardiovascular Health Engineering,N.T,Hong Kong,China [4]Department of Biomedical Engineering,City University of Hong Kong,Hong Kong,China [5]HUST-Suzhou Institute for Brainsmatics,Suzhou,China [6]Department of Mechanical and Automation Engineering,The Chinese University of Hong Kong,Shatin,N.T.,Hong Kong,China [7]School of Biomedical Engineering,Hainan University,Haikou,China

出　　处：《Light(Science & Applications)》2025年第1期47-74,共28页光(科学与应用)(英文版)

基　　金：supported by the National Science and Technology Innovation 2030(Grant No.2021ZD0201001);the National Natural Science Foundation of China(Grant Nos.62325502,81827901);Research Grants Council(Grant No.C5031-22GF),and InnoHK Centre(Grant No.COCHE-1.5).

摘　　要：In recent advancements in life sciences,optical microscopy has played a crucial role in acquiring high-quality three-dimensional structural and functional information.However,the quality of 3D images is often compromised due to the intense scattering effect in biological tissues,compounded by several issues such as limited spatiotemporal resolution,low signal-to-noise ratio,inadequate depth of penetration,and high phototoxicity.Although various optical sectioning techniques have been developed to address these challenges,each method adheres to distinct imaging principles for specific applications.As a result,the effective selection of suitable optical sectioning techniques across diverse imaging scenarios has become crucial yet challenging.This paper comprehensively overviews existing optical sectioning techniques and selection guidance under different imaging scenarios.Specifiically,we categorize the microscope design based on the spatial relationship between the illumination and detection axis,i.e.,on-axis and off-axis.This classification provides a unique perspective to compare the implementation and performances of various optical sectioning approaches.Lastly,we integrate selected optical sectioning methods on a custom-built off-axis imaging system and present a unique perspective for the future development of optical sectioning techniques.

关 键 词：ILLUMINATION INTENSE INTEGRATE 

分 类 号：O47[理学—半导体物理]