Structure and genome editing activity of the novel CRISPR-Cas12o1 effector  

作　　者：Zhiqiang Duan Xi Zhang Jun-Tao Zhang Xingkun Ji Ruiheng Liu Ying Chen Shanshan Li Nannan Jia Huizhi Gao Yu Xin Ning Jia Jian-Kang Zhu 

机构地区：[1]Institute of Advanced Biotechnology and School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong,China [2]Department of Biochemistry,School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong,China [3]Bellagen Biotechnology Co.Ltd.,Jinan,Shandong,China [4]Shenzhen Key Laboratory of Cell Microenvironment,Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research,Southern University of Science and Technology,Shenzhen,Guangdong,China [5]Key University Laboratory of Metabolism and Health of Guangdong,Institute for Biological Electron Microscopy,Southern University of Science and Technology,Shenzhen,Guangdong,China [6]Center for Advanced Bioindustry Technologies,Chinese Academy of Agricultural Sciences,Beijing,China

出　　处：《Cell Research》2025年第2期145-148,共4页细胞研究(英文版)

基　　金：supported by Shandong BellaGen Biotechnology Co.,Ltd.,and by grants from the National Natural Science Foundation of China(32188102 to J.-K.Z.and 32270050 to Ning J.);the Guangdong and Shenzhen Natural Science Foundation(2024A1515010541 and JCYJ20220530114409022 to Ning J.);the Guangdong Provincial Science and Technology Innovation Council Grant(2017B030301018).

摘　　要：Dear Editor,Class 2 type V CRISPR-Cas12 effectors exhibit tremendous diversity,utilizing either single crRNAs or dual RNA guides to target double-stranded DNA(dsDNA),single-stranded DNA(ssDNA)or RNA.For dsDNA targets,Cas12 recognizes different PAM sequences and generates sticky ends on the target dsDNA.1 This characteristic enhances the efficiency of homology-directed repair2 and causes less off-target effects compared to SpCas9.3 Recent studies have identified a series of Cas12 nucleases,ranging from Cas12a to Cas12n,1,4 thereby greatly expanding the gene editing toolkit.

关 键 词：thereby utilizing EDITING 

分 类 号：R59[医药卫生—内科学]