Antifungal activity of curcumin-mediated photodynamic inactivation against Fusarium graminearum on maize  

作　　者：Shuliang Dong Lu Chen Shaojie Li Konglong Feng Guang Liu Hao Dong Guizhi Xu Huajian Ou Yang Liu Yong Zhao Jingjing Wang 

机构地区：[1]Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing,Foshan University,Foshan 528225,China [2]College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China [3]Shanghai Sixth People’s Hospital Affiliated to Shanghai JiaoTong University,Shanghai 201306,China [4]Sericultural&Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods,Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610,China [5]College of Light Industry and Food Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China [6]National Technical Center(Foshan)for Quality Control of Famous and Special Agricultural Products,Foshan 528225,China

出　　处：《Grain & Oil Science and Technology》2025年第1期21-31,共11页粮油科技(英文版)

基　　金：supported by the National Key Research and Development Program of China(2022YFE0139500);National Natural Science Foundation of China(32102105);Guangdong Basic and Applied Basic Research Foundation(2024A1515013026;2023A1515140136);Special Projects in Key Areas of Higher Education Institution in Guangdong Province(2024ZDZX2090);Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing(2022B1212010015)。

摘　　要：The natural curcumin-mediated photodynamic inactivation(PDI)was developed,and its inactivation potency against Fusarium graminearum in vitro and in vivo was systematically investigated by fluorescence probe assay,trypan blue staining,scanning electron microscope(SEM),confocal laser scanning microscopy(CLSM),etc.Results showed that under the irradiation of blue LED,the photosensitizer of curcumin was excited to generate massive reactive oxygen species(ROS)in the cells of F.graminearum,and the PDI completely inactivated their mycelia and spores under the treatment of 150μM curcumin and 10.8 J/cm^(2)irradiation.Further analysis found that the PDI ruptured the cellular microstructures,damaged the cell membrane by increasing its permeability and oxidizing the lipids,degraded the intracellular DNA and proteins inside the spores of F.graminearum.Meanwhile,the PDI also potently killed>99.99%spores of F.graminearum on maize under the treatment of 200μM curcumin and 10.8 J/cm^(2)irradiation.Moreover,the PDI suppressed the production of zearalenone(ZEN),and residual ZEN could not be detected after the storage of maize for 10 days.Therefore,this study systematically explored the inactivation efficiency of curcumin-mediated PDI against both the mycelia and spores of F.graminearum,which provides a valid and promising method to control the fungal hazards in grains.

关 键 词：Fusarium graminearum Photodynamic inactivation(PDI) SPORES MYCELIA Zearalenone(ZEN) 

分 类 号：TS213.4[轻工技术与工程—粮食、油脂及植物蛋白工程]