聚乙二醇-硫酸铵双水相体系萃取桑叶总黄酮工艺研究  

Study on the Extraction of Total Flavonoids from Mulberry Leaves in PEG-600/(NH 4)2SO 4 Aqueous Two-phase System

作　　者：徐美 臧宇晴 李煦 范小振 XU Mei;ZANG Yuqing;LI Xu;FAN Xiaozhen(College of Chemistry and Chemical Engineering,Cangzhou Normal University,Cangzhou,Hebei 061001,China)

机构地区：[1]沧州师范学院化学与化工学院,河北沧州061001

出　　处：《沧州师范学院学报》2025年第1期33-37,共5页Journal of Cangzhou Normal University

基　　金：2023年度河北省高层次人才资助项目“桑葚有效成分的提取及微胶囊化研究”,编号:C20231002;2021年度沧州师范学院校内基金资助项目,编号:XNJJLYB2021005;2019年度沧州师范学院“天然产物研究与功能材料创新团队”,编号:cxtdl1901。

摘　　要：以桑科植物桑的叶子为原料,采用超声波-微波联合辅助聚乙二醇-硫酸铵双水相体系萃取桑叶中的总黄酮,探究了硫酸铵质量分数、萃取温度、萃取时间等不同单因素对总黄酮萃取得率的影响,并利用正交实验进行工艺优化。结果表明:当硫酸铵质量分数为21%、萃取温度为40℃、萃取时间为15 min时,桑叶总黄酮萃取得率可达到14.30 mg/g。桑叶总黄酮具有一定的抗氧化性,但总抗氧化能力比相同浓度的抗坏血酸(VC)要弱。Using the leaves of the mulberry plant as raw materials,a combined ultrasonic-microwave assisted polyethylene glycol(PEG-600)-ammonium sulfate aqueous two-phase extraction system was employed to extract the total flavonoids from mulberry leaves.The effects of different single factors such as the mass fraction of ammonium sulfate,extraction temperature,and extraction time on the extraction rate of total flavonoids were investigated,and the process was optimized using orthogonal experiments.The results showed that when the mass fraction of ammonium sulfate was 21%,the extraction temperature was 40℃,and the extraction time was 15 minutes,the extraction rate of total flavonoids from mulberry leaves could reach 14.30 mg/g.The total flavonoids from mulberry leaves have certain anti-oxidative properties,but their overall antioxidant capacity is weaker than that of ascorbic acid(VC)at the same concentration.

关 键 词：桑叶 总黄酮 超声波-微波 双水相体系 

分 类 号：TS201.1[轻工技术与工程—食品科学]