拟南芥根中细胞器特异标记蛋白质的定位观察  

Localization of Organelle-specific Marker Proteins in Arabidopsis thaliana Roots

作　　者：徐萌 王亚楠 李婷婷[1] 赵新英[1] XU Meng;WANG Ya-nan;LI Ting-ting;ZHAO Xin-ying(State Key Laboratory of Wheat Improvement/College of Life Sciences/Shandong Agricultural University,Tai'an 271018,China)

机构地区：[1]山东农业大学生命科学院,小麦育种全国重点实验室,山东泰安271018

出　　处：《山东农业大学学报(自然科学版)》2025年第1期125-132,共8页Journal of Shandong Agricultural University:Natural Science Edition

基　　金：拟南芥RAB5调控花粉管生长的功能和机理研究(32100296)。

摘　　要：构成细胞的微小结构或分区统称为亚细胞结构。每个亚细胞结构中都分布着特异的蛋白质,人们通过这些特异蛋白质与未知蛋白的共定位技术了解未知蛋白的功能。共定位技术中各细胞器的分布及形态观察需要相应的细胞器标记物来指示,然而鲜少有文献对细胞器标记物的定位进行系统全面的报道。本文通过共聚焦显微镜对各细胞器特异标记蛋白的定位情况进行观察,记录各细胞器在细胞内的分布情况,同时对于形态相似的细胞器通过药剂处理和利用超分辨成像技术进行进一步区分,以期在后续研究中为未知蛋白质的定位情况提供参考。The tiny structures or compartments that make up a cell are collectively referred to as subcellular structures.Each subcellular structure contains specific proteins.The function of unknown proteins can be inferred through co-localization techniques with these specific proteins.The distribution and morphology observation of each organelle in co-localization technology requires corresponding organelle markers to indicate.However,there are few systematic and comprehensive reports on the localization of organelle-specific marker proteins in the literature.In this paper,we observed the localization of organellespecific marker proteins in various organelles through confocal microscopy and recorded the distribution of each organelle in the cell,A further differitiention was made among the plesiomorphic Organelles with chemical treatment and super-resolution imaging.This paper was aimed to provide a reference for the localization of unknown proteins in subsequent studies.

关 键 词：细胞器特异标记蛋白质 亚细胞定位 细胞器 共聚焦显微镜 

分 类 号：Q246.6[生物学—细胞生物学]