基于网络药理学的附子泻心汤“异病同治”慢性萎缩性胃炎、慢性肾衰竭和复发性口腔溃疡的作用机制研究  

Exploring the Mechanisms of Fuzi Xiexin Decoction's“Different Diseases,Same Treatment”for Chronic Atrophic Gastritis,Chronic Renal Failure,and Recurrent Oral Ulcers,Based on Network Pharmacology Approach

作　　者：黄梅 肖娟 徐智 张兴丽 李江 余晓玲 东方梓涵 Huang Mei;Xiao Juan;Xu Zhi;Zhang Xingli;Li Jiang;Yu Xiaoling;Dongfang Zihan(Traditional Chinese Medicine College,Yunnan University of Chinese Medicine,Kunming 650500,China;Department of Pharmacy,The Third Hospital Affiliated to Yunnan University of Chinese Medicine(Kunming Municipal Hospital of Traditional Chinese Medicine),Kunming 650599,China;College of Chemistry,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500 [2]云南中医药大学第三附属医院(昆明市中医医院)药学部,云南昆明650599 [3]昆明理工大学化学学院,云南昆明650500

出　　处：《亚太传统医药》2025年第3期154-160,共7页Asia-Pacific Traditional Medicine

基　　金：国家自然科学基金项目(82360844);云南省科技厅科技计划项目(202101AZ070001-173,202101AZ070001-126)。

摘　　要：目的:基于中医“异病同治”理论,探讨附子泻心汤(FZXXD)治疗慢性萎缩性胃炎(CAG)、慢性肾衰竭(CRF)、复发性口腔溃疡(ROU)的潜在作用机制。方法:运用TCMSP数据平台获取FZXXD的化学成分及靶点,检索GeneCards等数据库提取CAG、CRF和ROU的靶点,利用Venny软件得出成分和疾病交集靶点。采用Cytoscape 3.7.2制作FZXXD治疗CAG、CRF和ROU的成分-靶点-疾病网络分布图,通过String平台构建FZXXD交集靶点的蛋白质-蛋白质相互作用(PPI)网络,基于BioGPS数据平台和Cytoscape 3.7.2构建“FZXXD关键靶点-器官组织”网络分布图,借助DAVID数据库进行GO功能和KEGG通路富集分析。结果:共获得FZXXD治疗CAG、CRF和ROU的交集靶点69个。蛋白互作网络筛选出Akt1、TNF等可能是FZXXD治疗CAG、CRF和ROU的关键靶点。GO功能共富集644个条目,主要涉及基因表达正调控、细胞凋亡过程的负调控等因素。KEGG共富集144条通路,包括TNF、IL-17等信号通路。分子对接显示,芦荟大黄素、β-谷甾醇等活性成分与TP53、TNF等核心靶点对接结果良好。结论:FZXXD主要通过槲皮素、β-谷甾醇等核心成分作用于AKT、TNF等核心靶点,通过调控TNF和IL-17等信号通路,体现“异病同治”的作用,为附子泻心汤“异病同治”提供理论依据。Objective:To explore the potential molecular mechanism of the Fuzi Xiexin Decoction(FZXXD)in treating chronic renal failure(CRF),chronic atrophic gastritis(CAG)and recurrent oral ulcer(ROU)on the basis of the traditional Chinese medicine theory of“treating different diseases with the same treatment”.Methods:The TCMSP data platform was utilized to obtain the active ingredients and targets of FZXXD.OMIM and GeneCards databases were utilized to acquire diseases related proteins,and Venny software was utilized to identify the intersection points of components and diseases.Cytoscape 3.7.2 software was employed to draw the components-targets-diseases network diagram of FZXXD for treating CAG,CRF and ROU.String platform was constructed the protein-protein interaction(PPI)networks of intersection targets for FZXXD.BioGPS online software was employed to obtain and map the network diagram of“key target-organ tissue of FZXXD”.DAVID database was utilized to execute KEGG pathway enrichment analysis and GO functional enrichment analysis.AutoDock tools software was utilized to confirm molecular docking.Results:69 intersection targets of the FZXXD for CAG,CRF and ROU were obtained.Results of the protein interaction network analysis indicated that Akt1 and TNF may be the key targets of FZXXD for the treatment of CAG,CRF and ROU.644 items were enriched in GO function,mainly involving negative regulation of apoptotic process,positive regulation of gene expression and other causes.144 pathways were enriched in KEGG,including tumor necrosis factor signaling pathway,Interleukin IL-17 signaling pathway,etc.Active ingredients such as quercetin and beta-sitosterol were in good docking with the core targets such as TP53 and TNF.Conclusion:FZXXD acts on Akt and TNF through quercetin and β-sitosterol,regulates TNF and IL-17 signaling pathways,and reflects the role of“Treating different diseases with the same treatment”,which offers new proofs for the theory of“Treating different diseases with the same treatment”in tradition

关 键 词：慢性萎缩性胃炎 慢性肾衰竭 复发性口腔溃疡 附子泻心汤 异病同治 网络药理学 分子对接 

分 类 号：R289.5[医药卫生—方剂学]