糖尿病大鼠创面组织的靶向能量代谢组学研究  

Targeted energy metabolomics study on wound tissue of diabetic rats

作　　者：王晓阳 扈煜婕 王晓川[2] 赵洁[1] 张基勋[2] 姜笃银[1] Wang Xiaoyang;Hu Yujie;Wang Xiaochuan;Zhao Jie;Zhang Jixun;Jiang Duyin(Emergency Medicine Center,the Second Hospital of Shandong University,Jinan 250033,China;Department of Plastic and Burns Surgery,the Second Hospital of Shandong University,Jinan 250033,China)

机构地区：[1]山东大学第二医院急诊医学中心,济南250033 [2]山东大学第二医院整形烧伤外科,济南250033

出　　处：《中华烧伤与创面修复杂志》2025年第2期137-144,共8页Chinese Journal of Burns And Wounds

基　　金：国家自然科学基金面上项目(81873934);国家自然科学基金青年科学基金项目(82202454);王正国创伤医学基金会生长因子复兴计划(SZYZ-TR-09);山东省科技发展计划(2015GSF118041);山东省自然科学基金青年基金项目(ZR2020QH168);济南市科技计划(202225065)。

摘　　要：目的 探讨糖尿病大鼠创面组织能量代谢变化.方法 该研究为实验研究.将6只8周龄的雄性SD大鼠按照随机数字表法分为造成糖尿病全层皮肤缺损创面的糖尿病组和仅造成全层皮肤缺损创面的对照组,每组3只.计算2组大鼠术后14 d创面愈合率.取2组大鼠术后14d创面组织,借助色谱-质谱分析进行靶向能量代谢组学检测,筛选2组大鼠创面组织间比较,表达量有显著变化的差异能量代谢物并对差异能量代谢物进行京都基因与基因组百科全书富集分析.结果 术后14 d,糖尿病组大鼠创面愈合率为(68.3±2.8)%,显著低于对照组的(98.1±1.2)%(t=16.92,P<0.05).术后14d,与对照组相比,糖尿病组大鼠创面组织中顺乌头酸、烟酰胺腺嘌呤二核苷酸磷酸、5'-鸟苷二磷酸表达量均显著升高(t值分别为4.74、3.09、3.99,P<0.05),L-苹果酸、乳酸表达量均显著降低(t值分别为3.45、12.20,P<0.05);2组大鼠创面组织中另外21种能量代谢物表达量比较,差异均无统计学意义(P>0.05). 5种差异能量代谢物主要在胰高血糖素、Rap1、Ras、缺氧诱导因子1信号通路及三羧酸循环、丙酮酸代谢、线粒体自噬、胞吞作用途径中显著富集.结论 糖尿病大鼠创面组织中乳酸及L-苹果酸表达量降低,顺乌头酸、烟酰胺腺嘌呤二核苷酸磷酸、5'-鸟苷二磷酸表达量升高;差异能量代谢物主要在氧化应激、能量调控、炎症反应等相关信号通路或途径中富集。ObjectiveTo explore the change of energy metabolism in wound tissue of diabetic rats.MethodsThis study was an experimental study.Six 8-week-old male Sprague-Dawley rats were divided into diabetic group inflicted with a diabetic full-thickness skin defect wound and control group only inflicted with a full-thickness skin defect wound according to the random number table method,with 3 rats in each group.The wound healing rates of rats in the two groups were calculated at 14 d after operation.The wound tissue of two groups of rats was collected at 14 d after operation,the targeted energy metabolomics detection was carried out by chromatography-mass spectrometry analysis,and the differential energy metabolites in the wound tissue were screened with significant change in the expression between the two groups of rats.The Kyoto encyclopedia of genes and genomes enrichment analysis was performed on the differential energy metabolites.ResultsAt 14 d after operation,the wound healing rate of rats in diabetic group was(68.3±2.8)%,which was significantly lower than(98.1±1.2)%in control group(t=16.92,P<0.05).At 14 d after operation,compared with those in control group,the expressions of cis-aconitic acid,nicotinamide adenine dinucleotide phosphate,and 5'-guanosine diphosphate were significantly increased in wound tissue of rats in diabetic group(with t values of 4.74,3.09,and 3.99,respectively,P<0.05),the expressions of L-malic acid and lactic acid were significantly decreased(with t values of 3.45 and 12.20,respectively,P<0.05),and there were no statistically significant differences in the expression levels of the other 21 energy metabolites in wound tissue of rats in the two groups(P>0.05).The five differential energy metabolites were enriched mainly in the glucagon,Rap1,Ras,hypoxia-inducible factor 1 signaling pathways as well as the tricarboxylic acid cycle,pyruvate metabolism,mitochondrial autophagy,and endocytosis pathways.ConclusionsThe expressions of lactic acid and L-malic acid in wound tissue of diabetic rats decre

关 键 词：创伤和损伤 糖尿病 能量代谢 柠檬酸循环 乳酸 L-苹果酸 

分 类 号：R28[医药卫生—中药学]