基于生物学信息分析TM7SF2在结直肠癌中的表达及预后  

Expression and prognostic significance of TM7SF2 in colorectal cancer based on bioinformatics analysis

作　　者：简莹 王仲琪 张志 张雪梅 JIAN Ying;WANG Zhongqi;ZHANG Zhi;ZHANG Xuemei(School of Public Health,North China University of Science and Technology,Tangshan 063210,Hebei,China;Affiliated Tangshan Gong Ren Hospital,North China University of Science and Technology,Tangshan 063000,Hebei,China;College of Life Science,North China University of Science and Technology,Tangshan 063210,Hebei,China;Hebei Key Laboratory of Occupational Health and Safety for Coal Industry,Tangshan 063210,Hebei,China)

机构地区：[1]华北理工大学公共卫生学院,河北唐山063210 [2]华北理工大学附属唐山市工人医院,河北唐山063000 [3]华北理工大学生命科学学院,河北唐山063210 [4]河北省煤矿卫生与安全重点实验室,河北唐山063210

出　　处：《中国现代医生》2025年第5期9-12,共4页China Modern Doctor

基　　金：河北省自然科学基金(H2023105018);河北省唐山市科技计划项目(23130232E)。

摘　　要：目的利用生物信息学技术分析跨膜7超家族成员2(transmembrane 7 superfamily member 2,TM7SF2)基因在结直肠癌中的表达及其与患者预后的关系。方法利用癌症基因组图谱数据库下载结直肠癌组织和正常组织的表达谱数据和临床资料,并对TM7SF2进行差异分析及临床相关性分析。通过单因素及多因素Cox回归分析,评估TM7SF2基因表达水平对患者预后的影响。下载基因集并用基因集变异分析(gene set variation analysis,GSVA)算法评分评估样本生物学功能变化;根据TM7SF2基因表达水平将患者分为高、低组,并用基因集合富集分析信号通路差异;运用CIBERSORT工具评估肿瘤免疫细胞浸润与TM7SF2关系;预测该基因的药物化疗敏感度,并分析TM7SF2基因表达与结直肠癌患者临床指标间的相关性。结果与正常组织比较,肿瘤组织的TM7SF2表达量上调,TM7SF2基因高表达与结直肠癌患者预后不良相关。通过单因素和多因素Cox回归模型分析,TM7SF2表达水平可作为结直肠癌患者预后的独立因素。通路富集分析结果显示,TM7SF2可能在DNA复制、Wnt信号通路及戊糖磷酸等途径中发挥作用。TM7SF2表达水平与调节性T细胞和CD8^(+)T细胞呈正相关,与记忆性CD4^(+)T细胞和中性粒细胞呈负相关。在药物敏感度方面,TM7SF2表达水平与喜树碱、顺铂、多西他赛、尼洛替尼、奥拉帕尼、阿昔替尼的敏感度具有相关性。结论TM7SF2在结直肠癌组织中高表达,与患者预后不良相关,可作为预后生物标志物。Objective To analyze the expression and prognostic association of transmembrane 7 superfamily member 2(TM7SF2)in colorectal cancer using bioinformatics techniques.Methods The differential expression levels and clinical relevance of TM7SF2 mRNA in normal tissues and colorectal tumor tissues were analyzed using The Cancer Genome Atlas(TCGA)database.Evaluate the impact of TM7SF2 gene expression levels on patient prognosis through univariate and multivariate Cox regression analysis.Gene sets were download and score biological function changes by using the gene set variation analysis algorithm.Patients were divide into high and low expression groups based on TM7SF2 gene expression levels and the differences in signaling pathways with gene set enrichment analysis(GSEA)analyzed;use the CIBERSORT tool to assess the relationship between tumor immune cell infiltration and TM7SF2,apply the genomics of drug sensitivity in cancer(GDSC)database to predict the drug chemosensitivity of this gene,and describe the association between TM7SF2 gene expression and clinical indicators in colorectal cancer patients.Results Compared with normal tissues,the expression level of TM7SF2 in tumor tissues was significantly upregulated.Survival analysis indicates that high expression of the TM7SF2 gene was associated with poor prognosis in colorectal cancer patients.Through univariate and multivariate Cox regression model analysis,it was found that the expression level of TM7SF2 was a risk factor for the prognosis of colorectal cancer.Enrichment analysis suggested that TM7SF2 may play a role in pathways such as DNA replication,Wnt signaling pathway,and pentose phosphate pathway.TM7SF2 was positively correlated with regulatory T cells and CD8^(+)T cells,and negatively correlated with memory CD4^(+)T cells and neutrophils.In terms of drug sensitivity,TM7SF2 is correlated with the sensitivity to Camptothecin,Cisplatin,Docetaxel,Nilotinib,Olaparib,and Axitinib.Regression analysis showed that the contribution of TM7SF2 expression distribution to dif

关 键 词：结直肠癌 预后 免疫浸润 药物敏感度 

分 类 号：R735.3[医药卫生—肿瘤]