FASTKDs在非小细胞肺癌预后中的潜在意义  

Potential significance of FASTKDs in prognosis of non-small cell lung cancer

作　　者：顾超 杨琪 GU Chao;YANG Qi(Department of Respiratory Medicine,the First Hospital of Jiaxing,Jiaxing 314000,Zhejiang,China)

机构地区：[1]嘉兴市第一医院呼吸内科,浙江嘉兴314000

出　　处：《中国现代医生》2025年第5期19-22,共4页China Modern Doctor

基　　金：浙江省医药卫生科技计划项目(2022KY377);嘉兴市科技计划项目(2021AD30177)。

摘　　要：目的分析非小细胞肺癌(non-small cell lung cancer,NSCLC)中Fas活化激酶结构域蛋白(fas-activated kinase domain-containing proteins,FASTKDs)的表达及与预后的相关性价值。方法通过分析癌症体细胞突变数据库中影响FASTKDs的体细胞突变情况。分析不同肺癌病理亚型中FASTKDs的mRNA表达水平。运用Kaplan-Meier生存分析评估FASTKDs在非小细胞肺癌样本中特异性表达的预测意义。采用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)和Western blot法分析FASTKDs在人NSCLC细胞中的mRNA和蛋白质表达情况。结果与正常肺组织相比,肺腺癌(lung adenocarcinoma,LUAD)组织中的所有FASTKDs均为高表达(P<0.05),而肺鳞癌组织中只有FASTKD1、FASTKD3、FASTKD4和FASTKD5表达升高(P<0.05)。在所有NSCLC及LUAD病理亚型患者中,只有FASTKD1和FASTKD3 mRNA表达水平升高与患者较高的总体生存期(overall survival,OS)相关(P<0.05),但在NSCLC患者中FASTKD2和FASTKD4 mRNA表达水平升高与患者OS的降低相关(P<0.05)。与正常人支气管上皮细胞相比,人NSCLC细胞中FASTKD1-5的mRNA和蛋白表达量增加(P<0.05)。结论不同的FASTKDs表达情况与NSCLC患者的预后相关,是NSCLC的潜在肿瘤标志物。Objective To analyze the correlation between the expression of fas-activated kinase domain-containing proteins(FASTKDs)and the prognosis of non-small cell lung cancer(NSCLC)and its predictive value.Methods By analyzing the somatic mutation in Catalog of Somatic Mutations in Cancer database,the effect of FASTKDs was analyzed.The mRNA expression level of FASTKDs in different pathological subtypes of lung cancer was analyzed retrospectively.Kaplan-Meier survival analysis was used to evaluate the predictive significance of FASTKDs specific expression in NSCLC samples.Real-time quantitative polymerase chain reaction(RT-qPCR)and Western blot were used to verify the mRNA and protein expression of FASTKDs in human NSCLC cells in vitro.Results FASTKDs in lung adenocarcinoma(LUAD)were highly expressed(P<0.05),while only FASTKD1,FASTKD3,FASTKD4 and FASTKD5 in lung squamous cell carcinoma were highly expressed(P<0.05).In all patients with NSCLC and LUAD pathological subtypes,only the increased expression levels of FASTKD1 and FASTKD3 mRNA were significantly correlated with the higher overall survival(OS)(P<0.05),but the increased expression levels of FASTKD2 and FASTKD4 mRNA in NSCLC patients were correlated with the decreased OS(P<0.05).Compared with normal human bronchial epithelial cells,the mRNA and protein expression of FASTKD1-5 in human NSCLC cells increased significantly(P<0.05).Conclusion Different expression of FASTKDs is related to the prognosis of NSCLC patients and is a potential tumor marker of NSCLC.

关 键 词：Fas活化激酶结构域蛋白 非小细胞肺癌 总体生存期 

分 类 号：R734.2[医药卫生—肿瘤]