木蝴蝶苷B在非小细胞肺癌顺铂耐药细胞系中的作用机制研究  

The mechanism of Oroxin B in cisplatin-resistant non-small cell lung cancer cell lines

作　　者：陈建 孟礼飞 汪俊 李晨蔚 胡文涛 CHEN Jian;MENG Lifei;WANG Jun;LI Chenwei;HU Wentao(The First Affiliated Hospital of Ningbo University,Ningbo 315010,Zhejiang,China)

机构地区：[1]宁波大学附属第一医院,宁波315010

出　　处：《现代实用医学》2025年第2期125-128,共4页Modern Practical Medicine

摘　　要：目的 探讨木蝴蝶苷B(OB)在顺铂耐药的非小细胞肺癌细胞系中的抗癌作用及其可能的分子机制。方法 以非小细胞肺癌细胞系A549和H1299为研究对象,通过增加顺铂剂量,持续暴露细胞,建立稳定的顺铂耐药细胞系A549/DDP和H1299/DDP。分别用OB (30μmol/L)及二甲基亚砜(DMSO,对照组)对A549/DDP和H1299/DDP进行处理。通过细胞计数试剂盒-8(CCK-8)实验检测细胞生存能力。采用Westernblot检测非小细胞肺癌细胞中的凋亡蛋白的相关表达。通过克隆形成实验检测顺铂耐药非小细胞肺癌细胞系的增殖能力。结果 OB表现出低剂量的细胞毒性。OB能抑制顺铂耐药细胞系A549/DDP和H1299/DDP的生存能力。Western blot结果显示OB能提高顺铂耐药细胞系A549/DDP和H1299/DDP中B淋巴细胞瘤-2基因(Bcl-2)相关蛋白X(Bax)、cleaved-caspase 3及cleaved-caspase 9的蛋白表达,降低Bcl-2、caspase9及caspase 3的蛋白表达(均P<0.05)。克隆形成实验结果显示OB可以明显抑制顺铂耐药细胞系中的细胞增殖能力。结论 OB对肺癌细胞仅有低剂量细胞毒性,但能过促进耐药肺癌细胞的凋亡和抑制耐药细胞的增殖能力,增强肺癌细胞对顺铂的敏感性。Objective To explore the anti-cancer effect of Oroxin B(OB)in cisplatin-resistant non-small cell lung cancer cell lines and its possible molecular mechanisms.Methods Non-small cell lung cancer(NSCLC)cell lines A549 and H1299 were used as the research objects.Stable cisplatin-resistant cell lines A549/DDP and H1299/DDP were established by increasing the dose of cisplatin and continuously exposing the cells.A549/DDP and H1299/DDP were treated with OB(30μmol/L)and DMSO(control group),respectively.The cell viability was detected by the cell counting kit-8(CCK-8)assay.Western blot was used to detect the related expression of apoptotic proteins in NSCLC cells.The proliferation ability of cisplatin-resistant NSCLC cell lines was detected by the colony formation assay.Re-sults OB showed low-dose cytotoxicity.OB could inhibit the viability of cisplatin-resistant cell lines A549/DDP and H1299/DDP.Western blot showed that OB could increase the protein expressions of B-cell lymphoma-2 gene(Bcl-2)-associated X protein(Bax),cleaved-caspase 3 and cleaved-caspase 9 in cisplatin-resistant cell lines A549/DDP and H1299/DDP,and decrease the protein expressions of Bcl-2,caspase 9 and caspase 3(all<0.05).The col-ony formation assay showed that OB could inhibit the cell proliferation ability in cisplatin-resistant cell lines signifi-cantly.Conclusions OB has low-dose cytotoxicity against lung cancer cells,but can promotes the apoptosis of drug-resistant lung cancer cells and inhibits the proliferation ability of drug-resistant cells,thereby enhancing the sensitivity of lung cancer cells to cisplatin.

关 键 词：木蝴蝶苷B 非小细胞肺癌 耐药 凋亡蛋白 

分 类 号：R734.2[医药卫生—肿瘤]