多组学分析MZT2A在皮肤黑色素瘤中的作用  

The role of MZT2A in skin cutaneous melanoma based on multiomics data

作　　者：徐子茜 林秉奖 邱勰 黄华 许素玲[1] 陆凌怡 XU Ziqian;LIN Bingjiang;QIU Xie;HUANG Hua;XU Suling;LU Lingyi(Department of Dermatology,the First Affiliated Hospital of Ningbo University,Ningbo 315010,Zhejiang,China;Department of Dermatology,Ningbo Medical Center Lihuili Hospital,Ningbo 315048,Zhejiang,China;Department of Rheumatology and Immunology,the First Affiliated Hospital of Ningbo University,Ningbo 315010,Zhejiang,China)

机构地区：[1]宁波大学附属第一医院皮肤科,浙江宁波315010 [2]宁波市医疗中心李惠利医院皮肤科,浙江宁波315048 [3]宁波大学附属第一医院风湿免疫科,浙江宁波315010

出　　处：《中国现代医生》2025年第6期11-18,共8页China Modern Doctor

基　　金：浙江省医药卫生科技计划项目(2022PY022)。

摘　　要：目的 基于多组学数据挖掘探究与评估有丝分裂纺锤体组织蛋白2A(mitotic spindle organizing protein 2A,MZT2A)与皮肤黑色素瘤(skin cutaneous melanoma,SKCM)的生存预后及免疫治疗反应之间的关联。方法 通过癌症基因组数据图谱、整合基因型组织表达、人类蛋白质图谱、SUMMER数据库,分析MZT2A在SKCM中的表达差异及生存预后。利用David2分析MZT2A生物学功能分析及相关富集通路。利用肿瘤免疫估计资源、肿瘤微环境单细胞数据库、肿瘤免疫系统互作、癌症图像档案和肿瘤免疫功能障碍及排除数据库分析和比较高低MZT2A基因表达组间免疫浸润细胞情况、免疫治疗反应及免疫逃逸能力的差异。结果 MZT2A基因在多种癌症中表达升高,尤其在SKCM中表达显著升高。高表达MZT2A的SKCM患者总体生存率降低。MZT2A表达量与SKCM样本中的CD8^(+)T细胞、CD4^(+)T细胞、巨噬细胞、中性粒细胞和树突状细胞的浸润程度均呈负相关。此外,MZT2A表达量越高,SKCM对于免疫检查点抑制剂(immune checkpoint inhibitors,ICI)的治疗反应可能越差。结论 MTZ2A基因的高表达与SKCM的不良预后相关,有望成为SKCM的生存预测靶标。高表达MTZ2A的SKCM患者对ICI治疗反应较差,以上对预测SKCM的生存预后、指导免疫靶向治疗提供理论依据。Objective To explore and evaluate the association between mitotic spindle organizing protein 2A(MZT2A)and survival prognosis and immunotherapy response of skin cutaneous melanoma(SKCM),based on multiomics data mining.Methods The expression difference and survival prognosis of MZT2A in SKCM were analyzed by the Cancer Genome Atlas database,Genotype-tissue Expression Data,Human Protein Atlas,SUMMER database.The biological function of MZT2A and related enriched pathways were analyzed by David2.The differences of immune infiltrating cells,immune therapy response and immune escape ability between high-and low-MZT2A groups were analyzed and compared by using Tumor Immune Estimation Resource,Tumor Immune Single-cell Hub,Tumor-immune System Interactions database,the Cancer Imaging Archive and Tumor Immune Dysfunction and Exclusion databases.Results The expression of MZT2A gene elevated in a variety of cancers,especially in SKCM.SKCM samples with high expression of MZT2A had a poor overall survival.The expression of MZT2A was negatively correlated with the infiltration of CD8^(+)T cells,CD4^(+)T cells,macrophages,neutrophils and dendritic cells in SKCM tissues.In addition,the higher the expression of MZT2A,the therapeutic response of SKCM were worse to immune checkpoint inhibitors(ICI).Conclusion MZT2A is a novel survival prediction target of SKCM.SKCM patients with high expression of MZT2A have poor response to ICI treatment,which provides a theoretical basis for predicting the survival prognosis and guiding immune targeted therapy of SKCM.

关 键 词：皮肤黑色素瘤 有丝分裂纺锤体组织蛋白2A 预后 多组学 免疫治疗 

分 类 号：R739.5[医药卫生—肿瘤]