不同取代度硫酸酯化裂褶多糖的制备及其降血糖和保湿活性  

Preparation of Sulfated Schizophyllan Polysaccharides with Different Degrees of Substitution and Their Hypoglycemic and Moisturizing Activities

作　　者：刘蔓萩 肖湲 伍磊 郭朝万 郑必胜[1] LIU Manqiu;XIAO Yuan;WU Lei;GUO Chaowan;ZHENG Bisheng(College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China;Guangdong Marubi Biotechnology Co.Ltd.,Guangzhou 510530,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640 [2]广东丸美生物技术股份有限公司,广东广州510530

出　　处：《现代食品科技》2025年第2期244-252,共9页Modern Food Science and Technology

基　　金：广东省重点领域研发计划项目(2022B1111080003)。

摘　　要：该研究探讨了以裂褶多糖为原料,采用三氧化硫-吡啶法制备硫酸酯化裂褶多糖以及取代度大小与其体外降血糖、体外保湿之间的关系。实验表明,随着三氧化硫-吡啶质量分数的增加,依次可获得取代度在0.66~1.81的4种硫酸酯化裂褶多糖。硫酸酯化裂褶多糖的重均分子量随着取代度的增加而逐渐降低,从1.67×10^(7) u下降到8.04×10^(5) u。取代度为1.19的硫酸酯化裂褶多糖PDI低至7.84,过度硫酸酯化将导致裂褶多糖内部发生无序降解。刚果红实验结果表明,硫酸酯化裂褶多糖不存在三螺旋结构。取代度为1.81的硫酸酯化裂褶多糖在质量浓度为2 mg/mL时,对α-葡萄糖苷酶抑制率可达73.8%,4种硫酸酯化裂褶多糖均为可逆混合I型抑制剂。硫酸酯化后裂褶多糖的吸湿与保湿活性均优于改性前,取代度为1.81的硫酸酯化裂褶多糖的吸湿能力可达77.93%(湿度43%)、52.54%(湿度81%),吸湿性仅次于甘油,但优于透明质酸。硫酸酯化裂褶多糖在干燥环境下的保湿性比透明质酸钠稍差。因此,硫酸酯化裂褶多糖具有的较好的α-葡萄糖苷酶抑制活性与吸湿保湿活性,两种活性均随着取代度的升高而增强。In this study,Schizophyllan Polysaccharides(SPG)was used as the as raw material,sulfated Schizophyllan polysaccharides(S-SPGs)was prepared by the sulfur trioxide-pyridine method,and the relationship between the degree of substitution(DS)of S-SPGs and their in vitro hypoglycemic and moisturizing activitieswas examined.The experimental results showed that four S-SPGs with a DS of 0.66~1.81 could be obtained sequentially with the increase of the mass fraction of sulfur trioxide-pyridine. The weight-average molecular weight Mw of S-SPGs decreased gradually from 1.67×10^(7) u to 8.04×10^(5) u with the increase of DS. The PDI of S-SPG with a DS of 1.19 was as low as 7.84. Excessive sulfation would lead to disordered degradation of S-SPGs. The results of Congo red experiment showed that there was no triple helix structure in S-SPGs. T The inhibitory rate of the S-SPG with a DS of 1.81 and concentration of 2mg/mL against α-glucosidase could reach 73.8%. All the four S-SPGs were reversible mixed type I inhibitors. The moisture-absorbing and moisture-holding capacities of the SPG after sulfation were superior to those before modification, and the moisture-absorbing capacities of the S-SPG with DS1.81 were 77.93% (RH 43%) and 52.54% (RH 81%), respectively, which were second only to glycerol but better than hyaluronic acid. In dry environments, S-SPGs had slightly worse moisture-holding capacities than sodium hyaluronate. Therefore, S-SPGs had better α-glucosidase-inhibitory activity and moisture-absorbing and moisture-holding capacities, and both of the latter capacities increased with the increase of substitution degree.

关 键 词：硫酸酯化裂褶多糖 降糖作用 保湿 

分 类 号：TS201.2[轻工技术与工程—食品科学]