牛乳铁蛋白对肺癌A549细胞增殖和凋亡的影响及其机制  

Effect of bovine lactoferrin on proliferation and apoptosis of lung cancer A549 cells and its mechanism

作　　者：苏津贤 舒星富 陈遥 赵钰[1,2] 张博文 张海霞 SU Jinxian;SHU Xingfu;CHEN Yao;ZHAO Yu;ZHANG Bowen;ZHANG Haixia(Key Bio-engineering and Technology Laboratory,Biomedical Research Center of the Northwest Minzu University,Lanzhou 730030,Gansu Province,China)

机构地区：[1]西北民族大学生物医学研究中心生物工程与技术国家民委重点实验室,甘肃兰州730030 [2]西北民族大学生命科学与工程学院,甘肃兰州730030

出　　处：《中国生物制品学杂志》2025年第2期149-154,共6页Chinese Journal of Biologicals

基　　金：甘肃省自然科学基金(23JRRA717);2023年度甘肃省教育厅高等学校创新基金(2023B-059);西北民族大学中央高校基本科研业务费专项资金资助项目(31920230165)。

摘　　要：目的探讨牛乳铁蛋白(bovine lactoferrin,bLF)对肺癌A549细胞增殖和凋亡的影响及其可能的机制,为bLF抗肺癌相关研究提供实验依据。方法使用不同浓度的bLF处理A549细胞,显微镜观察bLF对细胞形态的影响,CCK-8法检测其对细胞增殖活性的影响,克隆形成与划痕试验检测其对细胞增殖和迁移的影响,qRT-PCR和Western blot检测细胞凋亡相关基因和蛋白的表达情况。结果bLF对A549细胞生长具有明显的抑制作用,且呈浓度和时间依赖性。与0 mg/mL bLF组相比,经0.5与1.0 mg/mL bLF处理后的A549细胞集落形成能力均明显降低(t分别为6.016和30.24,P分别为0.0038和0.000),体外迁移能力逐渐减弱。此外,bLF可引起A549细胞凋亡信号通路的激活。与对照组相比,0.5与1.0 mg/mL bLF组细胞中的促凋亡蛋白Bax及P53的表达水平显著提高(Bax:t分别为6.454和11.11,P分别为0.0232和0.008;P53:t分别为6.962和49.46,P分别为0.0022和0.0004),抑凋亡蛋白Bcl-2表达水平显著降低(t分别为4.333和10.44,P分别为0.0494和0.0091),高浓度(1.0 mg/mL)bLF组可切割pro-caspase-3信号,激活caspase-3(t=5.759,P=0.0289)。结论bLF可显著抑制肺癌A549细胞增殖,并促进其凋亡,从而发挥抗肿瘤作用。Objective To investigate the effect of bovine lactoferrin(bLF)on the proliferation and apoptosis of lung cancer A549 cells and its possible mechanism,so as to provide an experimental basis for the study of anti-lung cancer effect of bLF.Methods A549 cells were treated with different concentrations of bLF.The effect of bLF on cell morphology was observed under microscope,the effect on cell activity was detected by CCK-8,the effects on cell proliferation and migration were verified by clone formation and scratch assay,and the expression of apoptosis-related genes and proteins was determined by qRTPCR and Western blot.Results The bLF had a significant inhibitory effect on the growth of A549 cells in a concentrationand time-dependent manner.Compared with the 0 mg/mL bLF group,the colony formation ability of A549 cells treated with0.5 and 1.0 mg/mL bLF significantly decreased(t=6.016 and 30.24,P=0.0038 and 0.000,respectively),and the in vitro migration ability was gradually weakened.In addition,bLF induced the activation of apoptosis signaling pathway in A549cells.Compared with the control group,the expression levels of pro-apoptotic proteins Bax(t=6.454 and 11.11,P=0.0232and 0.008,respectively)and P53(t=6.962 and 49.46,P=0.0022 and 0.0004,respectively)significantly increased and the expression level of anti-apoptotic protein Bcl-2(t=4.333 and 10.44,P=0.0494 and 0.0091,respectively)significantly decreased in the 0.5 and 1.0 mg/mL bLF groups.The bLF at a high concentration of 1.0 mg/mL bLF group could cleave pro-caspase-3(t=5.759,P=0.0289)signaling and activate caspase-3.Conclusion This study showed that bLF can significantly inhibit the proliferation of A549 lung cancer cells and promote the apoptosis,thus exerting an anti-cancer effect.

关 键 词：牛乳铁蛋白 肺癌 增殖 凋亡 

分 类 号：R943[医药卫生—药剂学]