miRNA-383-5p靶向CIP2A对膀胱癌细胞增殖、侵袭、迁移、凋亡的影响  

Effects of miRNA-383-5p targeting CIP2A on the proliferation,invasion,migration and apoptosis of bladder cancer cells

作　　者：李小丽 曹苏娟[1] 胡小毛[1] 邓玉洁 唐莉婷 张忠山[1] LI Xiaoli;CAO Sujuan;HU Xiaomao;DENG Yujie;TANG Liting;ZHANG Zhongshan(Department of Oncology,Affiliated Hospital of Xiangnan University,Chenzhou 423000,China)

机构地区：[1]湘南学院附属医院肿瘤科,郴州423000

出　　处：《实用肿瘤学杂志》2025年第1期30-38,共9页Practical Oncology Journal

摘　　要：目的检测膀胱癌组织及膀胱癌细胞5637、BIU-87、TCCSUP、HT-1376中miR-383-5p表达情况,探讨miR-383-5p靶向CIP2A对膀胱癌细胞增殖、侵袭、迁移和凋亡的影响。方法选取人膀胱癌组织及对应的癌旁组织,人膀胱癌细胞5637、BIU-87、TCCSUP、HT-1376和人膀胱移行上皮细胞,采用qRT-PCR法检测miR-383-5p的表达情况,选取miR-383-5p表达较低的细胞BIU-87进行后续试验。将BIU-87细胞分为空白组(正常培养)、miR-383-5p NC组(转染miR-383-5p阴性对照)、miR-383-5p mimic组(转染miR-383-5p模拟物)以及miR-383-5p mimic+pc-CIP2A组(共转染miR-383-5p模拟物和CIP2A过表达质粒pc-CIP2A),采用CCK-8试剂盒检测各组BIU-87细胞活力,流式细胞仪检测细胞凋亡率,Transwell小室实验检测细胞侵袭能力,划痕实验检测细胞迁移能力,Western blot法检测凋亡相关蛋白(caspase-3、Bax)、侵袭相关(MMP-2、MMP-9)、CIP2A/PP2A相关蛋白的表达情况,双荧光素酶实验验证miR-383-5p与CIP2A的靶向关系。结果miR-383-5p在膀胱癌组织及膀胱癌细胞中呈低表达;与空白组相比,miR-383-5p mimic组BIU-87细胞的miR-383-5p水平显著升高(0.91±0.10 vs.1.67±0.24,P<0.01),CIP2A蛋白表达显著降低(1.32±0.17 vs.0.45±0.03,P<0.001),细胞活力、侵袭、迁移能力、侵袭相关蛋白(MMP-2、MMP-9)表达、Bcl-2蛋白表达均显著降低[(100.00±4.36)%vs.(32.15±2.65)%、(150.20±12.95)个vs.(82.35±7.01)个、(77.91±3.63)%vs.(46.12±2.54)%、1.02±0.11 vs.0.22±0.04、1.03±0.18 vs.0.21±0.04、1.01±0.14 vs.0.27±0.05,P<0.001],凋亡率、凋亡相关蛋白(caspase-3、Bax)、PP2A表达均显著升高[(14.02±2.29)%vs.(38.21±3.20)%、0.81±0.11 vs.1.78±0.24、0.83±0.12 vs.1.72±0.24、0.27±0.02 vs.0.95±0.16,P<0.001];与miR-383-5p mimic组相比,miR-383-5p mimic+pc-CIP2A组BIU-87细胞活力、侵袭、迁移能力、侵袭相关蛋白(MMP-2、MMP-9)表达、Bcl-2蛋白表达均显著升高[(32.15±2.65)%vs.(50.18±3.77)%、(82.35±7.01)个vs.(116.30±13.70)个、(Objective The aim of this study was to detect the expression of miR-383-5p in bladder cancer tissues and bladder cancer 5637 cells,BIU-87 cells,TCCSUP cells and HT-1376 cells,and to explore the effects of miR-383-5p on the proliferation,invasion,migration and apoptosis of bladder cancer cells by targeting CIP2A.Methods The expression of miR-383-5p was detected by qRT-PCR in human bladder cancer tissues and their corresponding adjacent tissues,5637 cells,BIU-87 cells,TCCSUP cells,HT-1376 cells,human bladder transitional epithelial cells.BIU-87 cells with low miR-383-5p expression were selected for subsequent experiments.BIU-87 cells were divided into the blank group(normal culture),miR-383-5p NC group(negative control,transfected with miR-383-5p negative control),miR-383-5p mimic group(transfected with miR-383-5p mimic),and miR-383-5p mimic+pc-CIP2A group(co-transfected with miR-383-5p mimic and CIP2A overexpression plasmid pc-CIP2A).CCK-8 kit was used to detect the viability of BIU-87 cells in each group;Flow cytometry was used to detect apoptosis of BIU-87 cells;Transwell assay was used to measure cell invasion ability of BIU-87 cells;Scratch assay was used to measure cell migration ability of BIU-87 cells;Western blot was used to determine the expression of proteins related to apoptosis,invasion(MMP-2,MMP-9),and CIP2A/PP2A in BIU-87 cells;The dual luciferase assay was used to verify the targeting relationship between miR-383-5p and CIP2A in BIU-87 cells.Results The expression of miR-383-5p was low in bladder cancer tissues and bladder cancer cells.Compared with the blank group,BIU-87 cells in the miR-383-5p mimic group showed a significant increase the level of miR-383-5p(0.91±0.10 vs.1.67±0.24,P<0.01)and a significant decrease in the expression of CIP2A protein(1.32±0.17 vs.0.45±0.03,P<0.001),the cell viability,invasion,migration abilities,the expression of proteins related to invasion(MMP-2,MMP-9),and the expression of Bcl-2 protein[(100.00±4.36)%vs.(32.15±2.65)%,(150.20±12.95)vs.(82.35±7.01),(77.91±

关 键 词：miR-383-5p CIP2A 膀胱癌 增殖 侵袭 

分 类 号：R737.14[医药卫生—肿瘤]