基于微核技术探讨二氧化硫衍生物对小鼠遗传物质的损伤效应  

Effect of sulfur dioxide derivatives on micronucleus formation in mouse bone marrow cells

作　　者：付竞仪 厉海玥 王子昊 汪鑫 于文静 李海梅 FU Jingyi;LI Haiyue;WANG Zihao;WANG Xin;YU Wenjing;LI Haimei(School of Life Science and Technology,Shandong Second Medical University,Weifang 261053,China;School of Psychology,Shandong Second Medical University,Weifang 261053,China)

机构地区：[1]山东第二医科大学生命科学与技术学院,山东潍坊261053 [2]山东第二医科大学心理学院,山东潍坊261053

出　　处：《山东第二医科大学学报》2024年第6期417-420,共4页Journal of Shandong Second Medical University

基　　金：潍坊市科技发展计划项目(项目编号:2021GX059,2022YX038);潍坊医学院大学生创新创业训练计划项目(项目编号:X2022423)。

摘　　要：目的探究二氧化硫体内衍生物对小鼠遗传物质的损伤,评估二氧化硫对生物遗传稳定性造成的影响。方法选择48只昆明种小鼠为研究对象,随机分为8组,每组6只,雌雄各半,染毒试剂为亚硫酸钠和亚硫酸氢钠混合物(摩尔比3∶1)。其中1-4组为剂量模块组,染毒剂量分别为150 mg/kg、350 mg/kg、550 mg/kg和750 mg/kg,5-7组为时间模块组,染毒时间分别为36 h、48 h和60 h,第8组为空白对照组。行方差分析和t检验,比较各组小鼠的嗜多染红细胞微核率,用Pearson法分析各指标之间的相关性。结果不同剂量组别之间微核率有明显差别(P<0.001),多重比较显示,随着染毒剂量的增加,在一定浓度范围内(<550 mg/kg)微核率随之增高(P<0.001);染毒后36、48、60 h后小鼠微核率均明显高于对照组(P<0.001),组内多重比较显示,随着染毒时间的延长,微核率呈现先下降再上升的趋势(P<0.001)。结论二氧化硫体内衍生物会导致小鼠嗜多染红细胞微核率增加,并与染毒剂量与时间呈正向相关。Objective To explore the damage of sulfur dioxide derivatives in vivo to the genetic material of mice and evaluate the impact of sulfur dioxide on the genetic stability of organisms.Methods Forty-eight Kunming mice were selected as the research objects and randomly divided into 8 groups,with 6 mice in each group(half male and half female).The exposure reagent was a mixture of sodium sulfite and sodium bisulfite(molar ratio 3∶1).Groups 1-4 were dose-module groups,with exposure doses of 150 mg/kg,350 mg/kg,550 mg/kg,and 750 mg/kg,respectively.Groups 5-7 were time-module groups,with exposure times of 36 h,48 h,and 60 h,respectively.Group 8 was the blank control group.Analysis of variance and t-tests were performed to compare the micronucleus rates of polychromatic erythrocytes among different groups of mice.The Pearson method was used to analyze the correlations between various indicators.Results There were significant differences in the micronucleus rates among different dose groups(P<0.001).Multiple comparisons indicated that within a certain concentration range(<550 mg/kg),the micronucleus rate increased with the increase in exposure dose(P<0.001).After 36,48,and 60 hours of exposure,the micronucleus rates of mice were significantly higher than those of the control group(P<0.001).Multiple comparisons within the groups showed that with the prolongation of exposure time,the micronucleus rate first decreased and then increased(P<0.001).Conclusion Sulfur dioxide derivatives in vivo can increase the micronucleus rate of polychromatic erythrocytes in mice and are positively correlated with the exposure dose and time.

关 键 词：亚硫酸钠 亚硫酸氢钠 微核 小鼠 

分 类 号：R394.6[医药卫生—医学遗传学]