丰富环境通过海马BDNF相关通路减轻社会隔离所致认知功能损伤  

Influence of enriched environment on cognitive impairment induced by social isolation through hippocampal BDNF-related pathways

作　　者：刘磊 王亚丽 胡景玉 LIU Lei;WANG Yali;HU Jingyu(Department of Science and Education,Hengshui People's Hospital,Hengshui,Hebei 053000,China;Department of Neurology,Hengshui People's Hospital,Hengshui,Hebei 053000,China;Clinical Laboratory,Hengshui People's Hospital,Hengshui,Hebei 053000,China)

机构地区：[1]衡水市人民医院科教处,河北衡水053000 [2]衡水市人民医院神经内一科,河北衡水053000 [3]衡水市人民医院检验科,河北衡水053000

出　　处：《安徽医药》2025年第4期687-691,I0002,共6页Anhui Medical and Pharmaceutical Journal

基　　金：河北医学科学研究重点课题计划项目(20181588)。

摘　　要：目的探究丰富环境对社会隔离所致认知功能损伤的影响及其与海马脑源性神经生长因子(BDNF)相关通路的关系。方法2023年1—4月,将30只小鼠按随机数字表法分为对照组(NC组)、模型组(SI组)和治疗组(SI+EE组),每组10只。NC组以每笼5只饲养于标准环境中,SI组和SI+EE组以每笼1只分别饲养于标准环境和丰富环境中,4周后检测小鼠认知功能和学习记忆能力;免疫组织化学荧光染色和蛋白质印迹法检测海马组织中BDNF和原肌球蛋白相关激酶B(TrkB)蛋白表达;逆转录聚合酶链式反应(RT-PCR)检测海马组织中BDNF和TrkB mRNA表达。结果NC组新事物分辨率为(32.68±5.55)%、新位置分辨率为(19.56±3.92)%、目标象限停留时间为(47.12±7.43)s、穿越平台次数为(4.63±0.54)次,均显著高于SI组的(10.89±3.57)%、(2.78±0.36)%、(30.92±2.95)s、(1.38±0.15)次,NC组逃避潜伏期显著低于SI组(P<0.05);和SI组相比,SI+EE组小鼠新事物分辨率、新位置分辨率、目标象限停留时间和穿越平台次数明显升高,逃避潜伏期明显降低(P<0.05)。NC组BDNF、TrkB mRNA表达量显著高于SI组的(P<0.05);和SI组相比,SI+EE组小鼠海马组织中BDNF、TrkB蛋白和mRNA表达明显升高(P<0.05)。结论丰富环境可减轻社会隔离所致小鼠的认知功能损伤,其可能和调节BDNF/TrkB信号转导通路有关。Objective Exploring the impact of enriched environment on cognitive impairment caused by social isolation and its rela-tionship with the hippocampal brain-derived neurotrophic factor(BDNF)related pathway.Methods From January 2023 to April 2023,30 mice were randomly assigned into a control group(NC group),a model group(SI group),and a treatment group(SI+EE group)according to numerical methods,each group consisting of 10 mice.The NC group was housed in groups of 5 in a standard environment,while the SI and SI+EE groups were individually housed in a standard and enriched environment,respectively.After 4 weeks,cognitive function and learning and memory abilities of the mice were assessed.Immunofluorescence staining and Western blot analysis were used to detect BDNF and Tropomyosin-related kinase B(TrkB)protein expression in hippocampal tissue.Reverse transcription poly-merase chain reaction(RT-PCR)was used to measure BDNF and TrkB mRNA expressions in hippocampal tissue.Results In the NC group,the novel object recognition,novel location recognition,target quadrant dwell time and times across the platform in NC group were significantly higher than those in SI group.[(32.68±5.55)%vs.(10.89±3.57)%,(19.56±3.92)%vs.(2.78±0.36)%,(47.12±7.43)s vs.(30.92±2.95)s,(4.63±0.54)times vs.(1.38±0.15)times,respectively].The escape latency in NC group was significantly shorter than that in SI group(P<0.05).Compared with the SI group,the SI+EE group showed significantly increased performances in novel object recognition,novel location recognition,target quadrant dwell time and times across the platform,as well as decreased escape latency(P<0.05).The mRNA expression levels of BDNF and TrkB in NC group were significantly higher than those in SI group(P<0.05),while compared with SI group,the SI+EE group showed significantly increased protein and mRNA expression levels of BDNF and TrkB in hippocampal tissue(P<0.05).Conclusion Enriched environment can improve cognitive impairment in socially isolated mice,which may be related to the

关 键 词：丰富环境 海马 脑源性神经生长因子/原肌球蛋白相关激酶B信号通路(BDNF/TrkB信号通路) 社会隔离 认知功能受损 

分 类 号：R74[医药卫生—神经病学与精神病学]