结肠癌病人血清miR-20a、miR-615的表达水平与肿瘤恶性程度的相关性研究  

Correlation between the expression levels of serum miR-20a and miR-615 and the malignant degree of colon cancer patients

作　　者：李颖[1] 张晓茹 赵洪焕[1] 徐进霞 郑璇 韩素桂[1] LI Ying;ZHANG Xiaoru;ZHAO Honghuan;XU Jinxia;ZHENG Xuan;HAN Sugui(Department of Nuclear Medicine,Tangshan People's Hospital,Tangshan,Hebei 063000,China)

机构地区：[1]唐山市人民医院核医学检验科,河北唐山063000

出　　处：《安徽医药》2025年第4期824-827,共4页Anhui Medical and Pharmaceutical Journal

基　　金：河北省医学科学研究课题计划项目(20191611)。

摘　　要：目的探讨结肠癌病人血清微RNA-20a(miR-20a)、微RNA-615(miR-615)水平与肿瘤恶性程度的相关性。方法回顾性选取2021年8月至2022年12月唐山市人民医院收治的127例结肠癌病人为研究组,另选取同期该院收治的结肠良性疾病病人122例为对照组。Pearson法分析结肠癌病人血清miR-20a、miR-615与癌胚抗原(CEA)及糖类抗原-199(CA-199)水平的相关性;受试者操作特征曲线(ROC曲线)分析血清miR-20a、miR-615联合CEA、CA-199水平对结肠癌的诊断价值。结果与对照组比较,研究组血清miR-20a(1.19±0.33比1.06±0.32)、CEA[(7.73±2.55)μg/L比(4.70±1.79)μg/L]和CA-199[(14.82±4.01)U/mL比(9.84±3.21)U/mL]水平较高(P<0.05),而miR-615(0.87±0.36比1.06±0.32)下降(P<0.05)。结肠癌病人T分期、分化程度、淋巴结转移情况与血清miR-20a、miR-615表达水平有关(P<0.05)。Pearson相关性分析显示,结肠癌病人血清miR-20a与CEA、CA-199水平呈正相关(P<0.05),血清miR-615水平与CEA、CA-199水平呈负相关(P<0.05)。血清miR-20a、miR-615、CEA和CA-199水平单独诊断结肠癌发生的曲线下面积(AUC)分别为0.82[95%CI:(0.77,0.87)]、0.79[95%CI:(0.74,0.84)]、0.84[95%CI:(0.78,0.88)]和0.84[95%CI:(0.78,0.88)];四个指标联合诊断的AUC[0.96,95%CI:(0.93,0.98)]大于血清miR-20a(Z=5.44,P<0.001)、miR-615(Z=5.68,P<0.001)、CEA(Z=5.62,P<0.001)和CA-199(Z=5.37,P<0.001)水平单独诊断的AUC。结论结肠癌病人血清中miR-20a表达升高、miR-615表达降低,二者联合CEA和CA-199对结肠癌的诊断价值较高。Objective To investigate the correlation between serum microRNA-20a(miR-20a)and microRNA-615(miR-615)levels and the malignant degree of colon cancer patients.Methods A retrospective study was conducted on 127 colon cancer patients admitted to Tangshan People's Hospital from August 2021 to December 2022,and an additional 122 patients with benign colon diseases admitted to the same hospital during the same period were regarded as the benign control group.The correlation between serum miR-20a,miR-615 and carcinoembryonic antigen(CEA)and carbohydrate antigen-199(CA-199)levels in patients with colon cancer was analyzed by Pearson correlation coefficient.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of serum miR-20a,miR-615 combined with CEA and CA-199 levels in colon cancer.Results Compared with the benign control group,the study group had higher levels of serum miR-20a(1.19±0.33 vs.1.06±0.32),CEA[(7.73±2.55)μg/L vs.(4.70±1.79)μg/L],and CA-199[(14.82±4.01)U/mL vs.(9.84±3.21)U/mL](P<0.05),but lower miR-615(0.87±0.36 vs.1.06±0.32)(P<0.05).The expression levels of miR-20a and miR-615 in serum were correlated with T stage,differentiation degree and lymph node metastasis in patients with colon cancer(P<0.05).Pearson correlation analysis showed that serum miR-20a was positively correlated with CEA and CA-199 levels(P<0.05),while serum miR-615 was negatively correlated with CEA and CA-199 levels(P<0.05).The areas under the curve(AUC)of serum miR-20a,miR-615,CEA and CA-199 for the diagnosis of colon cancer alone were 0.82[95%CI:(0.77,0.87)],0.79[95%CI:(0.74,0.84)],0.84[95%CI:(0.78,0.88)]and 0.84[95%CI:(0.78,0.88)];The AUC of the four indicators combined for the diagnosis[0.96,95%CI:(0.93,0.98)]was higher than that of serum miR-20a(Z=5.44,P<0.001),miR-615(Z=5.68,P<0.001),CEA(Z=5.62,P<0.001)or CA-199(Z=5.37,P<0.001)alone.Conclusions The expression level of miR-20a is increased and that of miR-615 is decreased in the serum of colon cancer patients.Their combination with CEA and CA

关 键 词：结肠肿瘤 微RNA-20a 微RNA-615 癌胚抗原 糖类抗原-199 诊断价值 

分 类 号：R735.35[医药卫生—肿瘤]