Molecularly imprinted fluorescence sensor chip for lactate measurement  

作　　者：Muersha Wusiman Fariborz Taghipour 

机构地区：[1]Chemical and Biological Engineering,University of British Columbia,Vancouver,Canada

出　　处：《Microsystems & Nanoengineering》2024年第6期189-201,共13页微系统与纳米工程(英文)

基　　金：financial support received from the Natural Sciences and Engineering Research Council of Canada(NSERC).

摘　　要：Lactate measurements provide an opportunity to conveniently evaluate bodily functions and sports performance.A molecularly imprinted fluorescence biochip provides an innovative way to achieve lactate measurement and overcomes the limitations of enzyme-based sensors.To realize this goal,ZnO quantum dots(QDs),a biocompatible sensing material,were combined with selective receptors comprised of molecularly imprinted polymers(MIPs).The lactate-selective imprinted polymers were formed using 3-aminopropyltriethoxysilane(APTES)and 5-indolyl boronic acid monomers.Furthermore,a new solid-phase sensing platform that overcomes the limitations of liquid-based sensors was developed to detect lactate in real-time.The platform consists of the biosensor chip with a thin-film sensing layer,an ultraviolet(UV)excitation source,and a portable light detector.The final sensor has a sensitivity of 0.0217 mmol L^(-1) for 0–30 mmol L^(-1) of lactate in phosphate-buffered saline(PBS)with a correlation coefficient of 0.97.The high sensor sensitivity and selectivity demonstrates the applicability of the ZnO QDs and synthetic receptors for sweat analysis.

关 键 词：OVERCOME LIMITATIONS BORON 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] O657.3[自动化与计算机技术—控制科学与工程]