Microfluidic impedance flow cytometer leveraging virtual constriction microchannel and its application in leukocyte differential  

作　　者：Minruihong Wang Jie Zhang Xiao Chen Yimin Li Xukun Huang Junbo Wang Yueying Li Xiaoye Huo Jian Chen 

机构地区：[1]State Key Laboratory of Transducer Technology,Aerospace Information Research Institute,Chinese Academy of Sciences,Beijing 100190,People’s Republic of China [2]School of Future Technology,University of Chinese Academy of Sciences,Beijing 100049,People’s Republic of China [3]CAS Key Laboratory of Genomic and Precision Medicine,Collaborative Innovation Center of Genetics and Development,Beijing Institute of Genomics,Chinese Academy of Sciences,Beijing 100101,People’s Republic of China [4]China National Center for Bioinformation,Beijing 100101,People’s Republic of China [5]School of Electronic,Electrical and Communication Engineering,University of Chinese Academy of Sciences,Beijing 100049,People’s Republic of China

出　　处：《Microsystems & Nanoengineering》2024年第6期493-503,共11页微系统与纳米工程(英文)

基　　金：supported by the Grants of 62331025 and 62121003 from the National Natural Science Foundation of China.

摘　　要：Microfluidic impedance flow cytometry has been widely used in leukocyte differential and counting,but it faces a bottleneck due to the trade-off between impedance detection throughput and sensitivity.In this study,a microfluidic impedance flow cytometer based on a virtual constriction microchannel was reported,in which the virtual constriction microchannel was constructed by crossflow of conductive sample and insulated sheath fluids with underneath micro-electrodes for impedance measurements.Compared to conventional mechanical constriction microchannels,this virtual counterpart could effectively avoid direct physical contact between cells and the microchannel walls to maintain high throughputs,and significantly reduce the volume of the impedance detection region for sensitivity improvements.Using the developed microfluidic impedance flow cytometer,impedance pulses of three leukemia cell lines,K562,Jurkat,and HL-60,were detected,achieving a 99.8%differentiation accuracy through the use of a recurrent neural network.Furthermore,impedance pulses of four white blood cell subpopulations(neutrophils,eosinophils,monocytes,and lymphocytes)from three donors were detected,achieving a classification accuracy of≥99.2%.A classification network model was established based on purified white blood cell and applied to impedance pulses of two white blood cell mixtures,resulting in proportional distributions of four leukocyte subpopulations within theoretical ranges.These results indicated that the developed microfluidic impedance flow cytometer based on the virtual constriction microchannel could achieve both high detection throughput and high sensitivity,showing great potentials for clinical diagnostics and blood analysis.

关 键 词：FLUID LEUKOCYTE IMPEDANCE 

分 类 号：R446.1[医药卫生—诊断学] Q812[医药卫生—临床医学]