牛樟芝磷酸葡萄糖异构酶(PGI)基因的克隆、表达及蛋白特性分析  

Analysis on the Cloning,Expression and Protein Characteristics of PGI Gene from Antrodia cinnamomea

作　　者：刘宇煌 陆鑫 林娟娟 钟小丽 颜巧玲 蒋咏梅[1] 章文贤[1] LIU Yu-huang;LU Xin;LIN Juan-juan;ZHONG Xiao-li;YAN Qiao-ling;JIANG Yong-mei;ZHANG Wen-xian(College of Life Science,Fujian Normal University,Fuzhou,Fujian 350007,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350007

出　　处：《福建农业科技》2025年第1期31-37,共7页Fujian Agricultural Science and Technology

基　　金：福建省高校产学研联合创新项目(2022N5004)。

摘　　要：磷酸葡萄糖异构酶(PGI)催化6-磷酸-葡萄糖向6-磷酸-果糖的转化,是多糖合成过程的重要分支节点。以灵芝PGI蛋白为探针,基于牛樟芝基因组数据库,采用电子克隆技术获得牛樟芝pgi基因,分析该基因编码蛋白质的特性,并通过RT-PCR检验基因在发酵过程中的转录表达水平。结果表明:牛樟芝pgi基因的cDNA长度为1659 bp,编码552个氨基酸,所编码的PGI蛋白具亲水性,无跨膜及信号肽结构,二级结构以47.83%α-螺旋为主,该蛋白序列与绣球菌和北方淀粉囊孔菌亲缘关系最近。在固体培养和液体培养牛樟芝过程中,随时间推进pgi基因转录表达水平提高,多糖含量则相反。研究结果可为牛樟芝多糖合成的代谢调控奠定基础。Glucose phosphate isomerase(PGI)catalyzes the conversion of 6-phosphate-glucose to 6-phosphatefructose,which is an important branch node in the process of polysaccharide synthesis.By using PGI protein of Ganoderma lucidum as a probe,based on the genome database of Antrodia cinnamomea,the pgi gene of Antrodia cinnamomea was cloned by using the electronic cloning technology,and the characteristics of the protein encoded by the gene were analyzed.The transcript expression level of the gene during the fermentation process was detected by RT-PCR.The results showed that the cDNA length of pgi gene was 1659 bp,encoding 552 amino acids,and the encoded PGI protein was hydrophilic and had no transmembrane and signal peptide structure.The secondary structure was dominated by 47.83%α-helix.The protein sequence had a close genetic relationship with Sparassis crispa and Amylocystis lapponica.In the process of solid culture and liquid culture of Antrodia cinnamomea,the level of pgi gene transcription expression increased with time,which was inversely proportional to the synthesis of polysaccharides.The results of this study could lay a foundation for the metabolic regulation of polysaccharide synthesis in Antrodia cinnamomea.

关 键 词：牛樟芝 磷酸葡萄糖异构酶 多糖 蛋白特性 

分 类 号：S567.3[农业科学—中草药栽培]