The implications of single-cell RNA-seq analysis in prostate cancer:unraveling tumor heterogeneity,therapeutic implications and pathways towards personalized therapy  

作　　者：De-Chao Feng Wei-Zhen Zhu Jie Wang Deng-Xiong Li Xu Shi Qiao Xiong Jia You Ping Han Shi Qiu Qiang Wei Lu Yang 

机构地区：[1]Department of Urology,Institute of Urology,West China Hospital,Sichuan University,Chengdu 610041,China [2]Division of Surgery&Interventional Science,University College London,London WC1E 6BT,UK

出　　处：《Military Medical Research》2025年第2期220-241,共22页军事医学研究(英文版)

基　　金：Chinese Scholarship Council(202206240086);National Natural Science Foundation of China(81974099,82170785,81974098,82170784);National Key Research and Development Program of China(2021YFC2009303);programs from Science and Technology Department of Sichuan Province(2021YFH0172);Young Investigator Award of Sichuan University 2017(2017SCU04A17);Technology Innovation Research and Development Project of Chengdu Science and Technology Bureau(2019-YF05-00296-SN);Sichuan University-Panzhihua science and technology cooperation special fund(2020CDPZH-4).

摘　　要：In recent years,advancements in single-cell and spatial transcriptomics,which are highly regarded developments in the current era,particularly the emerging integration of single-cell and spatiotemporal transcriptomics,have enabled a detailed molecular comprehension of the complex regulation of cell fate.The insights obtained from these methodologies are anticipated to significantly contribute to the development of personalized medicine.Currently,single-cell technology is less frequently utilized for prostate cancer compared with other types of tumors.Start-ing from the perspective of RNA sequencing technology,this review outlined the signifcance of single-cell RNA sequencing(scRNA-seq)in prostate cancer research,encompassing preclinical medicine and clinical applications.We summarize the differences between mouse and human prostate cancer as revealed by scRNA-seq studies,as well as a combination of multi-omics methods involving scRNA-seq to highlight the key molecular targets for the diagnosis,treatment,and drug resistance characteristics of prostate cancer.These studies are expected to provide novel insights for the development of immunotherapy and other innovative treatment strategies for castration-resistant prostate cancer.Furthermore,we explore the potential clinical applications stemming from other single-cell technologies in this review,paving the way for future research in precision medicine.

关 键 词：Prostate cancer Single-cell RNA sequencing(scRNA-seq) Tumor microenvironment Tumor heterogeneity Treatment resistance Precision medicine 

分 类 号：R73[医药卫生—肿瘤]