质谱法检测血液中免疫抑制剂的研究进展  

Advances in the Detection of Immunosuppressive Agents in Blood by Mass Spectrometry

作　　者：潘菲 谢洁 屈子裕 刘粮泽 易可可 张谛 方向[2] 江游[2] 叶子弘 PAN Fei;XIE Jie;QU Zi-yu;LIU Liang-ze;YI Ke-ke;ZHANG Di;FANG Xiang;JIANG You;YE Zi-hong(College of Life Sciences,China Jiliang University,Hangzhou 310000,China;Technology Innovation Center of Mass Spectrometry for State Market Regulation,Center for Advanced Measurement Science,National Institute of Metrology,Beijing 100029,China)

机构地区：[1]中国计量大学生命科学学院,浙江杭州310000 [2]中国计量科学研究院前沿计量科学中心,国家市场监管技术创新中心(质谱),北京100029

出　　处：《质谱学报》2025年第2期254-264,I0006,共12页Journal of Chinese Mass Spectrometry Society

基　　金：国家重点研发计划项目(2021YFC2401100);国家自然科学基金-国家重大科研仪器研制项目(21927812);国家市场监督技术创新中心(质谱)2024年度开放课题(AKYKF2408)。

摘　　要：免疫抑制剂主要用于对抗器官移植术后出现的排斥反应,具有降低患者发病率和死亡率的重要作用。然而,一些免疫抑制剂具有治疗范围窄、药动学个体差异大、药物间存在相互作用等特点。由于免疫抑制剂血药浓度与疗效和毒性密切相关,因此,检测血液中免疫抑制剂浓度以充分了解患者体内的药物暴露情况,优化给药剂量以维持稳定的血药浓度,对确保用药的有效性和安全性有着重要意义。质谱具有高灵敏度、高特异性、高选择性、快速、微量等特点。本文综述了液相色谱-串联质谱法和原位电离质谱法在免疫抑制剂血药浓度检测中的应用,并对质谱技术检测血液中免疫抑制剂浓度进行展望。Immunosuppressive drugs are mainly used to fight against immune rejection after organ transplantation,and have great potential to reduce the incidence rate and mortality of patients.However,some drugs have characteristics such as narrow therapeutic range,significant individual differences in pharmacokinetics,and interactions between drugs.The blood drug concentration is closely related to efficacy and toxicity.Therefore,detecting the concentration of immunosuppressants in the blood can fully understand the drug exposure in the patient's body,optimizing the dosage can maintain a stable blood drug concentration,which are of great significance in ensuring the effectiveness and safety of medication.Mass spectrometry,as an analytical tool,has the characteristics of high sensitivity,high specificity,high selectivity,high speed,and low sampleconsumption.This article reviewed the applications of liquid chromatography tandem mass spectrometry and in situ mass spectrometry in the detection of immunosuppressants in blood.Liquid chromatography tandem mass spectrometry has the characteristics of high sensitivity and good repeatability.In situ mass spectrometry can directly detect blood samples without the need for sample pretreatment.Finally,the prospect of mass spectrometry technology in the detection of immunosuppressants in blood was discussed.

关 键 词：免疫抑制剂 血药浓度监测 质谱 血液 

分 类 号：O657.63[理学—分析化学]