全氟辛烷磺酸对斑马鱼肠道形态结构及微生物菌群的影响  

PERFLUOROOCTANE SULFONATE(PFOS)ON INTESTINAL STRUCTURE AND MICROBIOTA OF ZEBRAFISH(DANIO RERIO)

作　　者：祁校羽 徐宏洲 龙静菲 闫晨阳 韩喜盼 刘海侠[1] QI Xiao-Yu;XU Hong-Zhou;LONG Jing-Fei;YAN Chen-Yang;HAN Xi-Pan;LIU Hai-Xia(College of Animal Sciences,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学动物科技学院,杨陵712100

出　　处：《水生生物学报》2025年第4期48-58,共11页Acta Hydrobiologica Sinica

基　　金：西北农林科技大学2021年大学生创新创业训练计划(202110712036)资助。

摘　　要：为探究全氟辛烷磺酸(Perfluorooctane sulfonate,PFOS)对鱼类的肠道毒性,将雌雄斑马鱼分别暴露于0、1和10 mg/L的PFOS中21d,通过存活率、肠道组织切片、16S rRNA高通量测序、实时荧光定量PCR等技术检测斑马鱼肠道形态结构及微生物菌群的变化。结果表明:暴露于10 mg/L PFOS的斑马鱼死亡率明显高于1 mg/L,且对雄鱼的影响大于雌鱼。1 mg/L浓度组雌雄鱼的存活率分别为93.3%和83.3%,而10 mg/L为33.3%和13.3%。1 mg/L PFOS暴露使斑马鱼的肠壁厚度轻微变薄、肠绒毛有破损的现象。10 mg/L浓度组肠壁厚度明显变薄,肠绒毛高度降低、肠黏膜上皮细胞肿胀并伴随严重的溶解现象。PFOS暴露21d后,肠道组织的炎症相关基因肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)和白介素10(IL-10),以及细胞凋亡相关基因胱天蛋白酶3(Caspase 3)、p53、B淋巴细胞瘤-2(Bcl2)的表达量均显著高于对照组,且Caspase3和p53基因在雄鱼的肠道的表达量显著高于雌鱼(P<0.05)。PFOS暴露显著增加了肠道菌群的多样性,改变了肠道菌群结构。厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)、放线菌门(Actinobacteriota)和拟杆菌门(Bacteroidota)的相对丰度均显著增加,而梭杆菌门(Fusobacteria)显著下降。在属水平,PFOS处理增加了罗尔斯通菌属(Ralstonia)和假单胞菌属(Pseudomonas)相对丰度,而降低鲸杆菌属(Cetobacterium)和气单胞菌属(Aeromonas)。两个处理组肠道菌中厚壁菌门/拟杆菌门(F/B)的比值高于对照组。PFOS处理增强了肠道菌群的氨基酸及脂代谢功能,但削弱了聚糖的合成与代谢能力。综上所述,PFOS长期暴露会引起水生动物的肠道结构损伤及微生物菌群失调,进而影响动物的健康。Perfluorooctane sulfonate(PFOS),as the main active component in anti-fouling agents used for textile and leather products,has been widely used in both industrial and daily settings.So far,PFOS contamination has been detected in groundwater,surface water,seawater,and even animal tissues.It exhibits notable toxicity to the liver,reproduction,development,and nervous system of fish;however,the effect on the fish intestine remain unclear.In this study,male and female zebrafish were exposed to 0,1,and 10 mg/L of PFOS for 21d,respectively,and the changes in intestinal morphology,structure,and microflora of zebrafish were detected by survival rate,intestinal tissue section,16S rRNA high-throughput sequencing,Real-time quantitative PCR,and other techniques.The results showed that zebrafish exposed to 10 mg/L PFOS had a significantly higher mortality rate than those exposed to 1 mg/L,with males being more affected than females.The survival rates of male and female fish in the 1 mg/L concentration group were 93.3%and 83.3%,while those in the 10 mg/L concentration group were 33.3%and 13.3%,respectively.Exposure to 1 mg/L PFOS led to a reduction in intestinal thickness and damage to intestinal villi.In the 10 mg/L group,the intestine was significantly thinner,villi height was reduced,and intestinal mucosal epithelial cells were swollen and accompanied by severe dissolution.After 21d PFOS exposure,the inflammation-related genes such as tumour necrosis factor-alpha(TNF-α),interleukin1beta(IL-1β),and interleukin10(IL-10),along with caspase3,p53,and B-cell lymphoma-2(Bcl2),were significantly higher than those of the control group.Furthermore,caspase3 and p53 expression in male intestines was significantly higher than those in females(P<0.05).PFOS exposure also significantly increased the diversity and altered the structure of gut microbiota,with a marked rise in the relative abundance of Firmicutes,Proteobacteria,Actinobacteriota,and Bacteroidota,and a significant decline in Fusobacteria.At the genus level,PFOS treatment increa

关 键 词：全氟辛烷磺酸(PFOS) 肠道结构 微生物菌群 斑马鱼 

分 类 号：X171.5[环境科学与工程—环境科学]