基于叶绿体基因组的中国水麦冬科系统发育研究  

PHYLOGENY OF JUNCAGINACEAE IN CHINA BASED ON COMPLETE CHLOROPLAST GENOME

作　　者：王璐瑶 周全 樊国有 袁庆 张晶晶 刘海瑞 WANG Lu-Yao;ZHOU Quan;FAN Guo-You;YUAN Qing;ZHANG Jing-Jing;LIU Hai-Rui(College of Eco-Environmental Engineering,Qinghai University,Xining 810016,China;Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;University of Chinese Academy of Sciences,Beijing 100049,China;Maixiu Forest Farm in Huangnan Tibetan Autonomous Prefecture in Qinghai Province,Huangnan 811399,China;Shijiazhuang Medical College,Shijiazhuang 050599,China)

机构地区：[1]青海大学生态环境工程学院,西宁810016 [2]中国科学院水生生物研究所,武汉430072 [3]中国科学院大学,北京100049 [4]青海省黄南藏族自治州麦秀林场,黄南811399 [5]石家庄医学高等专科学校,石家庄050599

出　　处：《水生生物学报》2025年第4期154-166,共13页Acta Hydrobiologica Sinica

基　　金：西宁市重大科技专项(2023-Z-13);中国科学院西部之光青年学者项目(xbzglzb2022043)资助

摘　　要：为探究水麦冬科(Juncaginaceae)系统发育上存在的争议,并丰富水麦冬科物种的遗传信息资源,研究对海韭菜(Triglochin maritima)和水麦冬(Triglochin palustris)叶绿体基因组进行测序、组装、注释和特征分析,并重建其与相关类群的系统发育关系。海韭菜和水麦冬叶绿体基因组呈环状四分体结构,全长分别为155881和155803 bp,分别鉴定出了57和60个SSRs及49个长重复序列。海韭菜和水麦冬叶绿体基因组偏好使用以A/U结尾的密码子。系统发育分析表明水麦冬科两个物种聚为一支,与其近缘类群遗传距离较远,于78.67 MYA与眼子菜属(Potamogeton)、虾海藻属(Phyllospadix)、大叶藻属(Zostera)、二药藻属(Halodule)、针叶藻属(Syringodium)、川蔓藻属(Ruppia)构成的分支产生分化。结合物种生境与形态特征支持将水麦冬属上升为水麦冬科。研究将不仅为探究水麦冬科叶绿体基因组结构进化奠定理论基础,也能为水麦冬科及相关类群物种保护和科学利用提供理论依据。To investigate the phylogeny of Juncaginaceae and enrich its genetic resources,the complete chloroplast genomes of Triglochin maritima and Triglochin palustris were sequenced,assembled,annotated,and characterized.In addition,the phylogenetic relationships of Juncaginaceae and closely related taxa were reconstructed.The results indicate that the chloroplast genomes of Triglochin maritima and Triglochin palustris exhibit circular structure with lengths of 155881 and 155803 bp,respectively,containing 57 and 60 SSRs and all 49 long repetitive sequences.In addition,Both genomes also show a preference for codons ending in A/U.Phylogenetic analysis shows that these two Juncaginaceae species form a single branch with a significant genetic distance from closely related taxa,diverging from Potamogeton,Phyllospadix,Zostera,Halodule,Syringodium,and Ruppia approximately 78.67 million years ago.In conjunction with the distributional habitat differences,this study supports the elevation of Triglochin to family status within Juncaginaceae.This study provides a theoretical foundation for investigating chloroplast genome structure evolution of Juncaginaceae and offers valuable insights for species conservation and scientific utilization of Juncaginaceae and its related taxa.

关 键 词：水麦冬科 叶绿体基因组特征 系统发育 分歧时间 

分 类 号：S567.239[农业科学—中草药栽培]