3D打印Gyroid结构TC4上颌骨仿生支架制备及表面微纳形貌的性能研究  

Preparation and performance evaluation of surface micro-nano morphology of 3D printed gyroid structured TC4 bionic maxillary bone scaffold

作　　者：陈贺 王心彧[1] 韩泽奎 王怀升 臧旖欣 刘璐 CHEN He;WANG Xin-yu;HAN Ze-kui;WANG Huai-sheng;ZANG Yi-xin;LIU Lu(Stomatology Collage of Jiamusi University,Experimental Center for Stomatological Engineering,Jiamusi University,Key Laboratory of Oral Biomedical Materials and Clinical Application,Jiamusi 154002,China)

机构地区：[1]佳木斯大学口腔医学院、佳木斯大学口腔医学工程实验中心、黑龙江省口腔生物医学材料及临床应用重点实验室,154002

出　　处：《中华老年口腔医学杂志》2025年第1期63-68,共6页Chinese Journal of Geriatric Dentistry

基　　金：黑龙江省自然科学基金项目(LH2022H089);黑龙江省教育厅基本科研业务费人才培养项目(2023-kyywf-0613)。

摘　　要：目的探索Gyroid结构试件表面构建微纳复合形貌对力学和生物学性能的影响,设计并制备修复上颌骨缺损的仿生支架。方法设计和制造壁厚0.25 mm、0.3 mm、0.35 mm、0.4 mm、0.45 mm的Gyroid结构力学模型,基于力学压缩试验筛选适用于修复上颌骨缺损的Gyroid结构壁厚,设计并通过激光选区熔化技术(selective laser melting,SLM)制备上颌骨修复支架。表面处理实验分组为SLM组(常规处理)、SLA组(Al_(2)O_(3)喷砂+酸处理)、SAN组(Al_(2)O_(3)喷砂+酸处理+阳极氧化),观测表面形貌、粗糙度、静态接触角,对比表面处理后各组弹性模量。将试件与小鼠胚胎成骨细胞前体细胞(MC3T3-E1)共培养,通过细胞增殖、细胞形态、碱性磷酸酶(alkaline phosphatase,ALP)活性分析试件对细胞增殖和分化的影响。结果0.35 mm壁厚Gyroid结构弹性模量为(2.78±0.03)Gpa,适用于上颌骨支架。同一壁厚下,表面处理前后试件弹性模量差异不具有统计学显著性(P>0.05)。SAN组的细胞增殖、细胞形态、ALP活性优于SLA组、SLM组(P<0.05)。结论0.35 mm壁厚的Gyroid结构经表面处理后对其力学性能影响不大,微纳复合形貌Gyroid结构表面具备较满意的细胞相容性和成骨效果。Objective To investigate the impact of constructing micro-nano composite morphology on the surface of Gyroidstructured specimens on their mechanical and biological properties,and to design and fabricate bionic scaffolds for repairing maxillary bone defects.Methods Mechanical models of Gyroid structures with wall thicknesses ranging from 0.25 mm to 0.45 mm,in increments of 0.05 mm,were designed and fabricated.Based on the results of mechanical compression tests,an optimal wall thickness for repairing maxillary bone defects was identified.Subsequently,a maxillary bone repair scaffold with the selected wall thickness was designed and manufactured using selective laser melting(SLM)technology.The surface treatment experiments were divided into SLM group(conventional treatment),SLA group(Al_(2)O_(3) sandblasting+acid treatment)and SAN group(Al_(2)O_(3) sandblasting+acid treatment+anodic oxidation).The surface morphology,roughness and static contact Angle were observed,and the elastic modulus of each group after surface treatment was compared.The samples were co-cultured with mouse embryonic osteoblast precursor cells(MC3T3-E1),and the effects of alkaline phosphatase(ALP)activity on cell proliferation and differentiation were analyzed.Results The elastic modulus of the 0.35 mm wall thickness Gyroid structure was(2.78±0.03)Gpa,which was suitable for the maxillary scaffold.Under the same wall thickness,the difference of elastic modulus before and after surface treatment was not statistically significant(P>0.05).The cell proliferation,cell morphology and ALP activity of SAN group were better than SLA group and SLM group(P<0.05).Conclusion The surface treatment of the Gyroid structure with a wall thickness of 0.35 mm has little effect on its mechanical properties.The micro-nano composite Gyroid structure has satisfactory cytocompatibility and osteogenic effect.

关 键 词：Gyroid结构 3D打印 上颌骨缺损 微纳复合结构 

分 类 号：R783.1[医药卫生—口腔医学]