High-precision colorimetric-fluorescent dual-mode biosensor for detecting acetylcholinesterase based on a trimetallic nanozyme for efficient peroxidase-mimicking  

作　　者：Meie Zheng Mingxing Liu Zichen Song Fei Ma Hongda Zhu Huiling Guo Hongmei Sun 

机构地区：[1]Cooperative Innovation Center of Industrial Fermentation(Ministry of Education&Hubei Province),National“111”Center for Cellular Regulation and Molecular Pharmaceutics,Key Laboratory of Fermentation Engineering(Ministry of Education),Hubei Key Laboratory of Industrial Microbiology,Hubei University of Technology,Wuhan 430068,China [2]Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables,College of Life Science and Technology,School of Mechanical Engineering,Hubei Engineering University,Xiaogan 432000,China

出　　处：《Journal of Materials Science & Technology》2024年第24期168-180,共13页材料科学技术(英文版)

基　　金：supported by the Hubei University of Technology Graduate Research Innovation Project(No.2022048);the Natural Science Foundation Project of Hubei Province(grant No.2022CFB533);the Scientific Research Plan of Education Department of Hubei Province(grant No.D20222702);the Natural Science Project of Xiaogan city(grant No.XGKJ20210100014).

摘　　要：Highly sensitive and stable acetylcholinesterase detection is critical for diagnosing and treating various neurotransmission-related diseases.In this study,a novel colorimetric-fluorescent dual-mode biosensor based on highly dispersive trimetal-modified graphite-phase carbon nitride nanocomposites for acetylcholinesterase detection was designed and synthesized by phosphorus doping and a mixed-metal MOF strategy.The specific surface area of trimetal-modified graphite-phase carbon nitride nanocomposites increased from 15.81 to 96.69 g m^(-2),and its thermal stability,interfacial charge transfer,and oxidation-reduction capability were enhanced compared with those of graphite-phase carbon nitride.First-principles density functional theory calculations and steady-state kinetic analysis are applied to investigate the electronic structures and efficient peroxidase-mimicking properties of trimetal-modified graphite-phase carbon nitride nanocomposites.The oxidation of 3,3',5,5'-tetramethylbenzidin was inhibited by thiocholine,which originates from the decomposition of thiocholine iodide by Acetyl-cholinesterase(AChE),resulting in changes in fluorescence and absorbance intensity.Due to the independence and complementarity of the signals,a highly precise colorimetric-fluorescent dual-mode biosensor with a linear range for detecting AChE of 4-20μU mL^(-1) and detection limits of 0.13μU mL^(-1)(colorimetric)and 0.04μU mL^(-1)(fluorescence)was developed.The spiking recovery of AChE in actual samples was 99.0%-100.4%.Therefore,a highly accurate,specific,and stable dual-mode biosensor is available for AChE detection,and this biosensor has the potential for the analysis of other biomarkers.

关 键 词：Graphitic carbon nitride Zeolitic imidazolate framework-8 Colorimetric-fluorescent dual-mode sensor Density functional theory calculations ACETYLCHOLINESTERASE 

分 类 号：Q503[生物学—生物化学] TB383[一般工业技术—材料科学与工程]