MEDPOR材料在睑外翻重建手术中的生物相容性评估及潜在细胞毒性研究  

Biocompatibility and cytotoxicity assessment of MEDPOR material in eyelid reconstruction surgery

作　　者：张宏彬 王慧星 冯聪颖 宋丽华 吴志中 史俊虎 白萍 ZHANG Hongbin;WANG Huixing;FENG Chongying;SONG Lihua;WU Zhizhong;SHI Junhu;BAI Ping(Hebei Institute of Ophthalmology,Hebei Eye Hospital,Xingtai 05400l,Hebei,China)

机构地区：[1]河北省眼科研究所,河北省眼科医院,河北邢台054001

出　　处：《中国病原生物学杂志》2025年第3期277-281,291,共6页Journal of Pathogen Biology

基　　金：河北省社会发展领域专项项目(No.2022ZC236)。

摘　　要：目的本研究旨在评估在睑外翻重建手术中使用的一种高密度多孔聚乙烯植入物(MEDPOR)的生物相容性。方法选取了河北省眼科医院,眼整形泪器科10例外翻性眼睑患者作为研究对象,同时选取5例与患者年龄匹配的屈光不正患者的泪液作为正常对照组。采用基于泪液蛋白质组学的方法,分析了患者术后3个月和12个月时泪液中的蛋白质组成,并通过Westernblot实验进行验证。通过体外角膜上皮细胞模型,使用MTT/CCK-8实验评估泪液处理后细胞的存活率。结果在睑外翻重建术后3个月(T3组)的患者泪液中,发现了6种与对照组存在差异的蛋白,其中S100A8蛋白在T3组的表达上调最显著。然而在睑外翻重建术后12个月(T12组)的患者泪液中,S100A8蛋白的表达水平与对照组无显著差异。MTT/CCK-8实验结果表明,T3组处理的角膜上皮细胞存活率显著低于对照组(P<0.01),T12组与对照组存活率差异无统计学意义(P>0.05)。结论MEDPOR材料在眼睑重建手术后可能会诱导一定程度的炎症反应,对角膜上皮细胞健康产生一定影响,但随着术后时间的增加,这种反应可能会逐渐消退。术后12个月时,泪液中的炎症标志物恢复正常,细胞存活率与对照组无显著差异,表明MEDPOR材料具有良好的长期生物相容性。Objective To evaluate the biocompatibility of a high-density porous polyethylene implant(MEDPOR)used in ectropion reconstruction surgery.Methods 10 patients with retrotropic eyelids from the Department of Oculoplastic Surgery at the Hebei Provincial Eye Hospital were selected as the research subjects.At the same time,the tears of 5 patients with refractive errors matching the patient's age were selected as the normal control group.A method based on tear proteomics was used to analyze the protein composition in the patients tears 3 months and 12 months after surgery,and verified by Western blot experiments.Using an in vitro corneal epithelial cell model,the MTT/CCK-8 assay was used to evaluate cell survival after tear treatment.ResultsIn the tears of patients 3 months after ectropion reconstruction(T3 group),6 proteins were found that were different from those in the control group,among which the expression of S100A8 protein was the most significantly up-regulated in the T3 group.However,in the tears of patients 12 months after ectropion reconstruction(T12 group),the expression level of S100A8 protein was not significantly different from that in the control group.The results of the MTT/CCK-8 experiment showed that the survival rate of corneal epithelial cells treated in the T3 group was significantly lower than that of the control group(P<0.01),and there was no significant difference in the survival rate between the T12 group and the control group(P>0.05).Conclusion MEDPOR material may induce a certain degree of inflammatory reaction after eyelid reconstruction surgery and have a certain impact on the health of corneal epithelial cells.However,this reaction may gradually subside as the postoperative time increases.At 12 months after surgery,inflammatory markers in tears returned to normal,and cell survival rates were not significantly different from those in the control group,indicating that the MEDPOR material has good long-term biocompatibility.

关 键 词：MEDPOR植入材料 睑外翻重建手术 生物相容性 泪液蛋白质组学 

分 类 号：R73[医药卫生—肿瘤]