紫花前胡素调节PI3K/AKT信号通路对流感病毒肺炎小鼠肺损伤的影响  

The effect of decursin on lung injury in influenza virus pneumonia mice by regulating the PI3K/AKT signaling pathway

作　　者：汤令群 贺新艳 许柏华 黄献 TANG Lingqun;HE Xinyan;XU Baihua;HUANG Xian(Department of Pediatrics,Guangzhou University of Chinese Medicine Dongguan Hospital,Dongguan Hospital of Traditional Chinese Medicine,Dongguan 523000,Guangdong,China)

机构地区：[1]广州中医药大学东莞医院(东莞市中医院)儿科,广东东莞523000

出　　处：《中国病原生物学杂志》2025年第3期292-296,共5页Journal of Pathogen Biology

基　　金：2024年度广东省中医药局中医药科研项目(No.20241347)。

摘　　要：目的技探究紫花前胡素(Dec)调节PI3K/AKT信号通路对流感病毒肺炎小鼠肺损伤的影响。方法将小鼠随机分为Control组、Model组、Dec低剂量(Dec-L)组、Dec高剂量(Dec-H)组、Dec-H+740Y-P(PI3K/Akt信号通路激活剂)组和达菲组。检测肺指数、肺指数抑制率、肺组织病毒载量;ELISA检测肺组织中IL-17、IL-6、IL-1O和TNF-α水平;HE染色观察肺组织形态;透射电镜观察小鼠肺组织超微结构;Westernblot检测小鼠肺组织中PI3K/AKT信号通路蛋白表达水平。结果Control组小鼠肺组织形态和超微结构正常;与Control组比较,Model组小鼠肺组织有明显损伤,肺泡细胞核膜、内质网线和线粒体结构异常,肺指数,肺组织病毒核酸表达水平、IL-17、IL-6和TNF-α水平、P-AKT/AKT和p-PI3K/PI3K蛋白表达水平升高,肺组织IL-10水平降低(P<0.05);与Model组比较,Dec-L组、Dec-H组和达菲组小鼠肺组织形态、肺泡细胞核膜、内质网和线粒体结构有明显改善,肺指数、肺组织病毒核酸表达水平、IL-17、IL-6和TNF-α水平、P-AKT/AKT和p-PI3K/PI3K蛋白表达水平降低,肺组织IL-10水平升高(P<0.05);740Y-P可降低Dec对流感病毒肺炎小鼠的改善作用(P<0.05);Dec-H组和达菲组小鼠上述各项检测指标处于同一水平(P>0.05)。结论Dec可降低流感病毒肺炎小鼠肺组织炎症,减轻肺组织损伤,可能与抑制PI3K/AKT信号通路有关.Objective To investigate the effect of decursin(Dec)on lung injury in influenza virus pneumonia mice by regulating the PI3K/AKT signaling pathway.Methods Mice were randomly assigned into Control group,Model group,Dec low-dose(Dec-L)group,Dec high-dose(Dec-H)group,Dec-H+740Y-P(PI3K/Akt signaling pathway activator)group,and Tamiflu group.Lung index,lung index inhibition rate,and lung tissue viral load were tested.ELISA was used to detect the levels of IL-17,IL-6,IL-10,and TNF-αin lung tissue.HE staining was used to observe the morphology of lung tissue.Observe the ultrastructure of mouse lung tissue by transmission electron microscopy.Detect the proteins expression levels of PI3K/AKT signaling pathway in mouse lung tissue by Western blot.Results The morphology and ultrastructure of lung tissue of mice in the Control group were normal.Compared with the Control group,the lung tissue of mice in the Model group showed obvious injury,and the nuclear membrane,endoplasmic reticulum,and mitochondrial structure of alveolar cells were abnormal,the lung index,viral nucleic acid expression levels in lung tissue,IL-17,IL-6,and TNF-αlevels,and p-AKT/AKT and p-PI3K/PI3K protein expression levels were higher,and the IL-10 level in lung tissue was lower(P<0.05).Compared with the Model group,the lung tissue morphology,alveolar cell nuclear membrane,endoplasmic reticulum,and mitochondrial structure of mice in the Dec-L group,Dec-H group,and Tamiflu group were obviously improved,the lung index,viral nucleic acid expression levels in lung tissue,IL-17,IL-6,and TNF-αlevels,and p-AKT/AKT and p-PI3K/PI3K protein expression levels were lower,and the IL-10 level in lung tissue was higher(P<0.05).740Y-P could reduce the improvement effect of Dec on influenza virus pneumonia mice(P<0.05).The abovementioned detection indicators of mice in Dec-H group and Tamiflu group were at the same level(P>0.05).Conclusion Dec can reduce lung tissue inflammation and alleviate lung tissue injury in mice with influenza virus pneumonia,which may be related

关 键 词：紫花前胡素 PI3K/AKT信号通路 流感病毒肺炎 肺损伤 

分 类 号：R73[医药卫生—肿瘤]