P21(WAF1/CIP1)蛋白表达与胃癌患者幽门螺杆菌感染的相关性分析  

Correlation analysis between P21(WAF1/CIP1)protein expression and Helicobacter pylori infection in patients with gastric cancer

作　　者：潘云鹤 张成鹏 高鹏 宋杨 PAN Yunhe;ZHANG Chengpeng;GAO Peng;SONG Yang(QiqiharFirstFactoryHospital,Department 7,Qiqihar 161005,Heilongjiang,China;Department of Gastroenterology,Qiqihar Jianhua Hospital;Emergency Department of Qiqihar Jianhua Hospital;Department 1 of General Surgery,Qiqihar Jianhua Hospital)

机构地区：[1]齐齐哈尔一厂医院内七科,黑龙江齐齐哈尔161005 [2]齐齐哈尔建华医院消化内科 [3]齐齐哈尔建华医院急诊内科 [4]齐齐哈尔建华医院普外一科

出　　处：《中国病原生物学杂志》2025年第4期439-444,共6页Journal of Pathogen Biology

摘　　要：目的探讨P21(WAF1/CIP1)蛋白表达与胃癌患者幽门螺杆菌感染的相关性及其与胃癌临床病理特征的关系。方法选取2023年1月至2024年6月确诊的62例胃癌患者作为病例组,48例非恶性胃病患者作为对照组。采用Western blot检测胃癌组织、癌旁正常组织和正常胃黏膜组织中P21蛋白的表达水平;通过快速尿素酶试验和^(13)C尿素呼气试验检测幽门螺杆菌感染状态。分析P21蛋白表达与幽门螺杆菌感染及临床病理特征之间的关系。结果胃癌组织中P21蛋白相对表达量显著高于正常胃黏膜组织(1.56±0.35vs0.98±0.22,t=10.152,P<0.001)。胃癌患者幽门螺杆菌感染阳性率为66.13%(41/62),高于对照组的43.75%(21/48),差异具有统计学意义(χ^(2)=5.675,P=0.017)。幽门螺杆菌阳性患者的P21蛋白表达高于阴性者(1.68±0.32vs1.34±0.28,t=4.335,P<0.001)。多因素Logistic回归分析显示,幽门螺杆菌感染(OR=3.85,95%CI:1.23-12.05,P=0.021)、淋巴结转移(OR=3.27,95%CI:1.06-10.12,P=0.040)和TNM分期Ⅲ-Ⅳ期(OR=3.46,95%CI:1.12-10.68,P=0.031)是P21蛋白高表达的独立影响因素。结论P21蛋白在胃癌组织中高表达,与幽门螺杆菌感染、淋巴结转移和晚期TNM分期密切相关。幽门螺杆菌感染可能通过上调P21蛋白表达,促进胃癌的发生发展。P21蛋白有望作为胃癌诊断和预后评估的潜在生物标志物。Objective To explore the correlation between P21(WAF1/CIP1)protein expression and Helicobacter pylori infection in gastric cancer patients and its relationship with the clinicopathological characteristics of gastric cancer.Methods 62 gastric cancer patients diagnosed from January 2023 to June 2024 were selected as the case group,and 48 patients with non-malignant gastric diseases were selected as the control group.Western blot was used to detect the expression level of P21 protein in gastric cancer tissue,adjacent normal tissue and normal gastric mucosa tissue;the infection status of Helicobacter pylori was detected by rapid urease test and ^(13)C urea breath test.Analyze the relationship between P21 protein expression,Helicobacter pylori infection and clinicopathological characteristics.Results The relative expression of P21 protein in gastric cancer tissue was significantly higher than that in normal gastric mucosa tissue(1.56±0.35 vs 0.98±0.22,t=10.152,P<0.001).The positive rate of Helicobacter pylori infection in gastric cancer patients was 66.13%(41/62),which was higher than 43.75%(21/48)in the control group.The difference was statistically significant(χ^(2)=5.675,P=0.017).The expression of P21 protein in H.pylori-positive patients was higher than that in negative patients(1.68±0.32 vs 1.34±0.28,t=4.335,P<0.001).Multivariate logistic regression analysis showed that H.pylori infection(OR=3.85,95%CI:1.23-12.05,P=0.021),lymph node metastasis(OR=3.27,95%CI:1.06-10.12,P=0.040)and TNM Stage II-IV(OR=3.46,95%CI:1.12-10.68,P=0.031)is an independent influencing factor for high expression of P21 protein.Conclusion P2l protein is highly expressed in gastric cancer tissues and is closely related to H.pylori infection,lymph node metastasis and advanced TNM staging.H.pylori infection may promote the occurrence and development of gastric cancer by upregulating the expression of P21 protein.P21 protein is expected to serve as a potential biomarker for the diagnosis and prognosis assessment of gastric cancer.

关 键 词：胃癌 P21蛋白 WAF1/CIP1蛋白 幽门螺杆菌 LOGISTIC回归分析 

分 类 号：R735.2[医药卫生—肿瘤]