Effect of electroacupuncture on the MMP-2/9 pathway mediated blood-brain barrier permeability in mice with Parkinson’s disease  

电针对帕金森病小鼠MMP-2/-9通路介导的血脑屏障通透性的影响

作　　者：Xiao-lei ZHANG Meng-ni HU Ya-nan LI Yao WANG Zhen RONG Jun MA 张小蕾;胡梦妮;李亚楠;汪瑶;荣臻;马骏(湖北中医药大学针灸骨伤学院/针灸治未病湖北省协同创新中心,武汉430060)

机构地区：[1]College of Acupuncture-Moxibustion and Orthopedics,Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture-moxibustion,Wuhan 430060,China

出　　处：《World Journal of Acupuncture-Moxibustion》2025年第1期58-65,共8页世界针灸杂志(英文版)

基　　金：Supported by National Natural Science Foundation of China:No.8147378。

摘　　要：Objective:To investigate the effects of electroacupuncture(EA)on the blood-brain barrier permeability and the regulation of matrix metalloproteinases(MMPs)in the mice with Parkinson’s disease(PD).Methods:Forty-eight C57BL/6 mice were randomly assigned into the normal control(NC)group,the PD model(PD)group,the EA group and the EA+SB-3CT inhibitor group(EA+SB-3CT),with 12 mice in each group.In this experiment,the PD model was established by intragastric administration(IG)with rotenone for 4 wk in the PD group,EA group and EA+SB-3CT group.In the EA+SB-3CT group,1 h after IG with rotenone,the mice were intraperitoneally injected with MMP-2/9 inhibitor,SB-3CT(25 mg/kg/d).After successfully modeled,in the EA group and EA+SB-3CT group,EA was conducted at“Fengfu(GV16)”and bilateral“Taichong(LR3)”and“Zusanli(ST36)”,at 1 mA and 2 Hz for 30 min each time,once a day,for consecutive 2 wk.The behavioral changes of the mice were observed in each group using the open field test,the level of tyrosine hydroxylase(TH)in the substantia nigra was determined by immunohistochemistry,the permeability of the blood-brain barrier was detected by Evans blue staining,and the protein expression of ZO-1,ocludin,claudin-1,MMP-2 and MMP-9 in the substantia nigra was detected by Western blotting.Results:Compared with the NC group,the behavioral scores increased(P<0.05),while total time of locomotion,total distance and average speed were reduced(P<0.05)in the PD group.The expression of TH in the substantia nigra decreased(P<0.05),Evans blue level in the brain tissue increased(P<0.05),and the protein expression of ZO-1,occludin and claudin-1 was lower(P<0.05),whereas MMP-2 and MMP-9 expression was higher(P<0.05)in the PD group.Compared with the PD group,behavioral scores decreased(P<0.05),while the total time of locomotion,total distance and average speed increased(P<0.05)in the EA group.Additionally,TH expression in the substantia nigra was elevated(P<0.05),Evans blue level in the brain tissue was lower(P<0.05),the protein expression

关 键 词：Parkinson’s disease ELECTROACUPUNCTURE Blood-brain barrier Tight junction protein MMP-2 MMP-9 

分 类 号：R73[医药卫生—肿瘤]