Effects of xiusanzhen acupuncture on hippocampal CA1 region damage and microglia activation in rats with vascular dementia:A trigeminal nerve perspective  

基于三叉神经的嗅三针对血管性痴呆大鼠海马CA1区损伤及小胶质细胞激活的影响

作　　者：Jie LI Qiang WANG Yuan WANG Jun-yang LIU Jie GUO Hua LI Hao-bin ZHANG Jie LIU An-ren ZHANG Hai-fa QIAO 李杰;王强;王渊;刘隽阳;郭婕;李华;张豪斌;刘捷;张安仁;乔海法(陕西中医药大学针灸推拿学院,陕西咸阳712046;陕西中医药大学陕西省针药结合重点实验室,陕西咸阳712046;陕西中医药大学第二临床医学院,陕西咸阳712046;加拿大不列颠哥伦比亚大学,加拿大温哥华V5K-V6Z;同济大学附属上海市第四人民医院,上海200434)

机构地区：[1]School of Acupuncture-Moxibustion and Tuina,Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi Province,China [2]The Second Clinical Medicine School,Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi Province,China [3]Key Laboratory of Acupuncture and Herbal Medicine of Shaanxi Province,Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi Province,China [4]University of British Columbia,Vancouver V5k-V6Z,Canada [5]Shanghai Fourth People’s Hospital Affiliated to Tongji University,Shanghai 200434,China

出　　处：《World Journal of Acupuncture-Moxibustion》2025年第1期75-83,共9页世界针灸杂志(英文版)

基　　金：Supported by The National Natural Science Foundation of China Young Scientists Fund:82004480。

摘　　要：Objective:This study aimed to observe the effects of xiusanzhen acupuncture(olfactory three-needling therapy)on the behavior,hippocampal histopathology and microglial(MG)activation of rats with vascular dementia(VD),and to assess the role of the trigeminal nerve.Methods:Sprague-Dawley(SD)rats were randomly assigned to the sham-operation,model,xiusanzhen,and trigeminal neurotomy groups(n=15 per group).Bilateral common carotid artery ligation was performed to prepare the VD models.Models of trigeminal neurotomy were prepared through the excision of the frontal nerve and infraorbital nerve.Xiusanzhen acupuncture was delivered via electric stimulation.The acupoints selected were bilateral“Yingxiang(LI20)”and“Yintang(EX-HN3)”,and the stimulation parameters were as follows:disperse-dense wave,at a frequency of 2/15HZ and intensity of 1 mA.The course of treatment was once daily,with one course lasting five days,followed by an interval of two days,yielding a total of four courses.Behavioral changes were detected using the Morris water maze,changes in histomorphology in the hippocampal CA1 region were determined with hematoxylin and eosin(HE)staining,and MG activation in the hippocampal CA1 region was detected using immunofluorescence.Results:The escape latency:From days 3 to 5,the escape latency was higher in the model group compared to the sham-operation group(P<0.05),but was lower in the xiusanzhen group compared to the model and trigeminal neurotomy groups(P<0.05).The frequency for platform crossing and swimming distance:both were reduced in the model group compared to the sham-operation group(P<0.01);and were elevated in the xiusanzhen group compared to the model group and trigeminal neurotomy group(P<0.01).Hippocampal pathomorphological changes:In the sham-operation group,the morphological structure and nucleoli were well-defined;in the model group and the trigeminal neurotomy group,cell numbers were reduced and karyopyknosis increased;while in the xiusanzhen group,the cell numbers were elevated and karyopyk

关 键 词：Trigeminal nerve Xiusanzhen acupuncture Vascular dementia MICROGLIA 

分 类 号：R24[医药卫生—中医临床基础]