甘草抑制猪流行性腹泻病毒感染的作用机制  

Mechanism of licorice in inhibiting porcine epidemic diarrhea virus infection

作　　者：张宗山 赵莹莹 顾明慧 苗钰松 李春秋[1] 邢晓旭 ZHANG Zongshan;ZHAO Yingying;GU Minghui;MIAO Yusong;LI Chunqiu;XING Xiaoxu(School of Animal Science and Technology,Heilongjiang Bayi Agricultural and Reclamation University,Daqing Heilongjiang 163319,China;School of Animal Science and Technology/School of Animal Medicine,Huazhong Agricultural University,Wuhan 430070,China;Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江·大庆163319 [2]华中农业大学动物科学技术学院、动物医学院,武汉430070 [3]黑龙江省农业科学院,哈尔滨150086

出　　处：《东北农业大学学报》2024年第8期133-141,共9页Journal of Northeast Agricultural University

基　　金：黑龙江省自然科学基金优秀青年基金项目(YQ2022C028);国家级大学生创新创业训练计划项目(202010223024)。

摘　　要：为初步探讨甘草抑制猪流行性腹泻病毒(PEDV)感染的活性成分及作用机制,利用网络药理学方法初步分析甘草抗PEDV的关键靶标和生物学过程,通过分子对接技术、荧光定量PCR验证甘草中活性成分与关键靶标潜在相互作用及对关键靶标基因表达的影响。结果表明:STAT3、TP53、IL-6和RELA等15个蛋白可能是甘草治疗猪流行性腹泻的关键靶标。分子对接显示甘草中活性成分可与关键位点产生结合,具有潜在相互作用。甘草提取物可显著下调STAT3、TP53和RELA等基因表达。To preliminarily investigate the active ingredients and mechanisms of action of licorice in inhibiting porcine epidemic diarrhea virus(PEDV)infection,network pharmacology methods were employed to analyze the key targets and biological processes associated with licorice's effects against PEDV.By molecular docking technology and fluorescence quantitative PCR,assessed potential interactions between the active ingredients in licorice and key targets,as well as their effects on gene expression.The results indicated that 15 proteins,including STAT3,TP53,IL-6,and RELA,might serve as key targets of licorice in the treatment of porcine epidemic diarrhea.Molecular docking studies demonstrated that the active ingredients in licorice could bind to critical sites,suggesting potential interactions.Furthermore,licorice extract significantly down-regulated the expression of genes such as STAT3,TP53,and RELA.

关 键 词：猪流行性腹泻病毒 甘草 网络药理学 分子对接 

分 类 号：Q812[生物学—生物工程] S855.3[农业科学—临床兽医学]