KIF14在宫颈癌组织中的表达及其对Hela细胞增殖和迁移的影响  

Expression of KIF14 in cervical cancer and its effect on proliferation and migration of Hela cells

作　　者：李映贤 莫颖禧 覃雪琪 莫凌昭 LI Ying-xian;MO Ying-xi;QIN Xue-qi;MO Ling-zhao(The Second Department of Gynecologic Oncology,Guangxi Medical University Cancer Hospital,Nanning 530000,Guangxi,CHINA;Department of Gynecology,Beihai Maternal and Child Health Hospital,Beihai 536000,Guangxi,CHINA)

机构地区：[1]广西医科大学附属肿瘤医院妇瘤二区,广西南宁530000 [2]北海市妇幼保健院妇科,广西北海536000

出　　处：《海南医学》2025年第5期609-615,共7页Hainan Medical Journal

基　　金：广西医疗卫生适宜技术开发与推广应用项目(编号:S2019041);广西医科大学附属肿瘤医院妇科优子项目(编号:2023-1号)。

摘　　要：目的 探讨驱动蛋白家族成员14 (KIF14)在宫颈癌中的表达及其对宫颈癌细胞系Hela细胞增殖、迁移能力的影响。方法 利用GEPIA2数据库、HPA数据库、TIMER2.0数据库分析KIF14于宫颈癌和正常组织中的表达差异以及相关临床意义。合成siRNA敲低Hela细胞中KIF14的表达,采用实时荧光定量PCR (RT-qPCR)检测Hela细胞siNC组和siKIF14组KIF14的m RNA表达水平,采用Western blot检测Hela细胞siNC组和siKIF14组KIF14的蛋白表达水平;采用CCK-8、细胞克隆形成实验检测细胞增殖能力;划痕实验、Transwell实验检测细胞迁移能力。结果 数据库分析结果显示KIF14在宫颈癌组织的mRNA水平和蛋白水平显著上调,且高表达KIF14的患者的无病生存期更短(P=0.044),但总生存期差异无统计学意义(P>0.05)。KIF14表达水平与CD8^(+)T细胞、巨噬细胞、M1型巨噬细胞和M2型巨噬细胞水平呈负相关(P<0.01)。Hela细胞siKIF14组的增殖速率较siNC组减慢,差异有统计学意义(P<0.01);Hela细胞siKIF14组的克隆形成数量较siNC组减少,siNC组为(154.33±12.10)个,siKIF14组为(112.33±8.62)个,差异有显著统计学意义(t=4.897,P<0.01)。Hela细胞siKIF14组在同样时间内划痕愈合率较si NC组减慢,si NC组为(56.60±13.71)%,si KIF14组为(28.61.00±9.06)%,差异有统计学意义(t=2.951,P<0.05);Hela细胞si KIF14组较si NC组穿过Transwell小室的细胞数量减少,si NC为(305.00±42.72)个,si KIF14组为(156.00±40.58)个,差异有统计学意义(t=4.380,P<0.05)。结论 KIF14在宫颈癌中表达上调,与肿瘤免疫相关,且可促进宫颈癌细胞增殖和迁移。KIF14具有作为预后和治疗标志物的潜力。Objective To investigate the expression of Kinesin family member 14(KIF14)and its effect on proliferation and migration ability of cervical cancer cell line Hela cells.Methods The expression differences and clinical significance of kinesin family member 14(KIF14)in cervical cancer and normal tissues were analyzed using the GEPIA2,HPA,and TIMER2.0 databases.siRNA was synthesized to knock down KIF14 expression in Hela cells.Real-time fluorescent quantitative PCR(RT-qPCR)and western blot were used to detect KIF14 mRNA and protein levels in siNC and siKIF14 groups.Cell proliferation was assessed using CCK-8 and colony formation assays,while cell migration was evaluated using wound healing and Transwell assays.Results Database analysis revealed that KIF14 was significantly upregulated at both mRNA and protein levels in cervical cancer tissues.Patients with high KIF14 expression had shorter disease-free survival(P=0.044),but no significant difference was found in overall survival(P>0.05).KIF14 expression negatively correlated with levels of CD8^(+)T cells,macrophages,M1 macrophages,and M2 macrophages(P<0.01).The proliferation rate of Hela cells in the siKIF14 group was significantly slower than in the siNC group(P<0.01).Colony formation was reduced in the siKIF14 group compared to the siNC group:(112.33±8.62)vs(154.33±12.10),t=4.897,P<0.01.Wound healing rates were slower in the siKIF14 group than in the siNC group:(28.61±9.06)%vs(56.60±13.71)%,t=2.951,P<0.05.Transwell migration assays showed fewer cells in the siKIF14 group compared to the siNC group:(156.00±40.58)vs(305.00±42.72),t=4.380,P<0.05.Conclusion KIF14 is upregulated in cervical cancer,correlates with tumor immunity,and promotes cervical cancer cell proliferation and migration.KIF14 has potential as a prognostic and therapeutic marker.

关 键 词：宫颈癌 驱动蛋白家族成员14 增殖 迁移 肿瘤免疫 

分 类 号：R737.33[医药卫生—肿瘤]