莲须的抗氧化效果及作用机制  

Antioxidant Effect and Mechanism of Action for Stamen Nelumbinis

作　　者：张蕾[1] 杨越 潘明月 徐萌 李小雨 吴禹岐 李金旭 ZHANG Lei;YANG Yue;PAN Mingyue;XU Meng;LI Xiaoyu;WU Yuqi;LI Jinxu(School of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,Heilongjiang Province,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011

出　　处：《吉林大学学报(理学版)》2025年第2期638-646,共9页Journal of Jilin University:Science Edition

基　　金：黑龙江省基本科研业务费项目(批准号:1453PT004;1453QN028);黑龙江省自然科学基金(批准号:LH2022C100);牡丹江师范学院项目(批准号:MNUQN202301;kjcx2023-119mdjnu)。

摘　　要：通过网络药理学和分子对接技术,研究莲须抗氧化的分子机制,并通过实验体外验证莲须清除自由基的能力.结果表明:莲须的抗氧化作用主要依赖于MAPKs信号通路和PI3K/AKT信号通路等生物过程调节;莲须对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)和羟基自由基(·OH)有一定的清除能力,当质量浓度为0.8 mg/L时,对DPPH·和·OH的清除率分别为83.6%和53.43%;高效液相色谱(HPLC)检测发现莲须中包含山奈酚、木犀草素和槲皮素等黄酮类化合物发挥抗氧化作用.We studied the molecular mechanism of antioxidative activity of stamen nelumbinis by network pharmacology and molecular docking technology,and verified the free radical scavenging ability of stamen nelumbinis through in vitro experiments.The results show that the antioxidant effects of stamen nelumbinis mainly relies on the regulation of biological processes such as MAPKs signaling pathway and PI3K/AKT signaling pathway.The stamen nelumbinis has a certain ability to clear 1,1-diphenyl-2-picrylhydrazyl radical(DPPH0·F)and hydroxyl radical(·OH),and the scavenging rates of DPPH·and·OH are 83.6%and 53.43%,respectively when the mass concentration is 0.8 mg/L.The high performance liquid chromatography(HPLC)detection reveals that the stamen nelumbinis contains flavonoids such as kaempferol,lignans,and quercetin,which exert antioxidant effects.

关 键 词：莲须 抗氧化 抑菌剂 网络药理学 分子对接 高效液相色谱分析 

分 类 号：Q819[生物学—生物工程]