膀胱癌组织中微小RNA-433-3p、β微管蛋白V的表达与病理特征的关系及对术后复发的预测价值  

Relationship between the expression of microRNA-433-3p and βV-tubulin in bladder cancer and pathological characteristics and their predictive value for postoperative recurrence

作　　者：杨林 韩玉刚 梁想 YANG Lin;HAN Yugang;LIANG Xiang(Department of Urology,Yiling Hospital,Hebei Province,Shijiazhuang 050090,China)

机构地区：[1]河北以岭医院泌尿外科,石家庄050090

出　　处：《临床外科杂志》2025年第2期175-178,共4页Journal of Clinical Surgery

摘　　要：目的 探讨膀胱癌组织中微小RNA-433-3p(miR-433-3p)、β微管蛋白V(βV-tubulin, TUBB6)的表达与病理特征的关系及对术后复发的预测价值。方法 2021年8月~2022年8月行手术治疗的膀胱癌病人癌组织及癌旁组织116例,根据是否出现术后局部复发或者远处复发转移分为复发组(43例)和未复发组(73例)。采用实时荧光定量PCR(qRT-PCR)检测miR-433-3p、TUBB6 mRNA水平;采用logistic回归分析膀胱癌病人术后复发的影响因素;受试者工作特征(ROC)曲线分析miR-433-3p、TUBB6对膀胱癌术后复发的预测价值。结果 癌组织中miR-433-3p水平显著低于癌旁组织,TUBB6水平显著高于癌旁组织,差异有统计学意义(P<0.05);miR-433-3p水平在TNM分期为Ⅲ+Ⅳ期、组织学分化为低分化、肿瘤浸润病人中明显降低,TUBB6 mRNA水平明显升高,差异有统计学意义(P<0.05);复发组病人血清miR-433-3p水平低于未复发组,TUBB6 mRNA水平高于未复发组,差异有统计学意义(P<0.05);TNM分期为Ⅲ+Ⅳ期、肿瘤浸润、TUBB6高表达为病人术后复发的独立危险因素(P<0.05),miR-433-3p高表达为独立保护因素(P<0.05);ROC曲线分析显示,血清miR-433-3p、TUBB6水平表达联合预测膀胱癌术后复发的曲线下面积(area under curve, AUC)明显大于单一指标预测(Z_(二者联合-miR-433-3p)=2.323、Z_(二者联合-TUBB6)=1.961,P=0.020、0.048),敏感度为95.35%,特异度为82.19%。结论 膀胱癌组织miR-433-3p、TUBB6表达与病理特征密切相关,二者联合对病人术后复发具有更高的预测价值。Objective To explore the relationship between the expression of microRNA-433-3p(miR-433-3p)andβmicrotubule V(TUBB6)in bladder cancer tissue and its pathological characteristics,and its predictive value for postoperative recurrence.Methods From August 2021 to August 2022,116 patients with bladder cancer underwent surgery for cancer tissue and adjacent tissue,and which were divided into recurrence group(43 cases)and non recurrence group(73 cases)according to whether there was local recurrence or distant recurrence and metastasis after surgery.The quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the mRNA levels of miR-433-3p and TUBB6.The influencing factors of postoperative recurrence of bladder cancer were analyzed by logistic regression.The predictive value of miR-433-3p and TUBB6 in postoperative recurrence of bladder cancer was analyzed by receiver operating characteristic(ROC)curve.Results The level of miR-433-3p in cancer tissue was obviously lower than that in adjacent tissue(P<0.05),and the level of TUBB6 was obviously higher than that in adjacent tissue(P<0.05).The level of miR-433-3p was significantly reduced(P<0.05)in patients with TNM stageⅢ+Ⅳ,histological differentiation to low differentiation,and tumor infiltration,while the level of TUBB6 mRNA was significantly increased(P<0.05).The serum miR-433-3p level in the recurrent group was significantly lower than that in the non recurrent group,,The level of TUBB6 mRNA was higher in the non recurrent group,and the difference was statistically significant(P<0.05).TNM stageⅢ+Ⅳ,tumor infiltration,and high expression of TUBB6 are independent risk factors for postoperative recurrence in patients(P<0.05),while high expression of miR-433-3p is an independent protective factor(P<0.05).ROC curve analysis shows that,,the area under curve(AUC)predicted by serum miR-433-3p and TUBB6 levels in combination was significantly higher than that predicted by a single index(Z-miR-433-3p=2.323,Z-TUB6=1.961,P=0.020,0.048),sensitivity of 95.35%

关 键 词：膀胱癌 微小RNA-433-3p β微管蛋白V 病理特征 术后复发 

分 类 号：R73[医药卫生—肿瘤]