百香果多糖对沙门氏菌感染雏鸡肠道免疫的调节作用  

Regulatory effects of passion fruit polysaccharides on intestinal immunity in chicks infected with Salmonella

作　　者：申心怡 黎媛媛 王晓霞 黄璟昇 黄康萍 李环 何家康[1] 梁正敏[1] SHEN Xinyi;LI Yuanyuan;WANG Xiaoxia;HUANG Jingsheng;HUANG Kangping;LI Huan;HE Jiakang;LIANG Zhengmin

机构地区：[1]广西大学动物科学技术学院,广西南宁530004

出　　处：《饲料研究》2025年第2期104-108,共5页Feed Research

基　　金：广西重点研发项目(项目编号:桂科AB23026082);广西科技基地和人才专项项目(项目编号:桂科AD23026289);广西大学大学生创新创业训练计划项目(项目编号:202310593440)。

摘　　要：试验旨在探究百香果多糖对感染沙门氏菌的雏鸡肠道黏膜免疫的调节作用。选取24只7日龄健康麻黄雏鸡随机分为正常对照组、感染对照组和百香果多糖组,每组8个重复,每个重复1只鸡。百香果多糖组雏鸡灌胃百香果多糖溶液5 d后经口感染沙门氏菌,感染24 h后采集十二指肠进行免疫指标检测和组织病理变化观察。结果显示,与感染对照组相比,百香果多糖组十二指肠灌洗液中免疫球蛋白G (IgG)和分泌型免疫球蛋白A(SIgA)水平显著升高(P<0.05),黏蛋白2 (MUC2)和三叶因子(TFF)水平略微升高,沙门氏菌诱导的小肠黏膜上皮结构损伤明显减轻。正常对照组和百香果多糖组的免疫器官指数、血清中谷胱甘肽过氧化物酶(GSH-Px)和总超氧化物歧化酶(T-SOD)活性无显著差异(P>0.05)。研究表明,百香果多糖可通过促进肠道免疫球蛋白分泌来改善沙门氏菌诱导的肠炎。The experiment aimed to investigate the immunomodulatory effect of passion fruit polysaccharides on the intestinal mucosa of Salmonella-infected ephedra chicks.A total of 24 healthy seven-day-old spotted-brown chicks were randomly divided into three groups:A normal control group,an infection control group,and a passion fruit polysaccharide group,with eight replicates in each group and one chicken in each replicate.The passion fruit polysaccharide group was gavaged with a passion fruit polysaccharide solution for five days and then orally infected with Salmonella.After 24 hours of infection,the duodenum was collected for immune marker detection and histological observation of tissue changes.The results showed that compared with the infection control group,passion fruit polysaccharide group significantly increased the levels of immunoglobulin G(IgG)and secretory immunoglobulin A(SIgA)in the duodenal wash fluid(P<0.05),slightly increased the levels of mucin 2(MUC2)and trefoil factor(TFF),and significantly alleviated the intestinal mucosal epithelial structural damage induced by Salmonella.There were no significant differences in the immune organ index,serum glutathione peroxidase(GSH-Px),and total superoxide dismutase(T-SOD)activities between the normal control group and passion fruit polysaccharide groups(P>0.05).The study indicates that passion fruit polysaccharides can improve Salmonella-induced enteritis by promoting the secretion of intestinal immunoglobulins.

关 键 词：百香果多糖 沙门氏菌 肠道免疫 免疫球蛋白 肠上皮损伤 

分 类 号：S816.7[农业科学—饲料科学]